Typical Testing Data/Standard Curve (for reference only)
Goat Lysophosphatidylcholine Acyltransferase 3 ELISA Kit | LPCAT3 elisa kit
Goat Lysophosphatidylcholine Acyltransferase 3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: LPCAT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LPCAT3 antibody and an LPCAT3-HRP conjugate. The assay sample and buffer are incubated together with LPCAT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LPCAT3 concentration since LPCAT3 from samples and LPCAT3-HRP conjugate compete for the anti-LPCAT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by LPCAT3 from the sample, fewer sites are left to bind LPCAT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPCAT3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
MBOAT5: Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3- phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero- 3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn- glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. Belongs to the membrane-bound acyltransferase family.
Protein type: Membrane protein, integral; EC 2.3.1.23; EC 2.3.1.n6; Membrane protein, multi-pass; Transferase
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane
Molecular Function: 1-acylglycerophosphocholine O-acyltransferase activity
Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process
Research Articles on LPCAT3
Similar Products
Product Notes
The Goat LPCAT3 lpcat3 (Catalog #AAA744708) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744708 ELISA Kit recognizes Goat LPCAT3. It is sometimes possible for the material contained within the vial of "Lysophosphatidylcholine Acyltransferase 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
