Canine Glycophorin A ELISA Kit | GYPA elisa kit

Canine Glycophorin A ELISA Kit

Cat. Num. AAA1603416

• Gene Names: GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• Reactivity: Canine
• Synonyms: Glycophorin A; Canine Glycophorin A ELISA Kit; GYPA elisa kit

• Reactivity: Canine
• Sequence Length: 150

• Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml-10ng/ml
• Sensitivity: 0.017ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GYPA elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Glucoside Xylosyltransferase 2 (also known as GXYLT2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine GXYLT2 antibody. GXYLT2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine GXYLT2 Antibody is added and binds to GXYLT2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GXYLT2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine GXYLT2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 298286506
• NCBI GeneID: 2993
• NCBI Accession #: NP_002090.4
• NCBI GenBank Nucleotide #: NM_002099.8
• UniProt Accession #: P02724
• NCBI Official Full Name: glycophorin-A isoform 1
• NCBI Official Synonym Full Names: glycophorin A (MNS blood group)
• NCBI Official Symbol: GYPA
• NCBI Official Synonym Symbols: MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• NCBI Protein Information: glycophorin-A
• UniProt Protein Name: Glycophorin-A
• Protein Family: Glycophorin
• UniProt Gene Name: GYPA
• UniProt Synonym Gene Names: GPA

NCBI Description

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

Uniprot Description

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).