Human LILRA3/CD85e ELISA Kit | LILRA3 elisa kit

Human LILRA3/CD85e ELISA Kit

Cat. Num. AAA1753891

• Gene Names: LILRA3; HM31; HM43; ILT6; LIR4; CD85E; ILT-6; LIR-4
• Reactivity: Human
• Synonyms: LILRA3/CD85e; Human LILRA3/CD85e ELISA Kit; HM31; HM43; ILT6; LIR4; CD85E; ILT-6; LIR-4; leukocyte immunoglobulin like receptor A3; LILRA3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human LILRA3

• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- a polyclonal antibody from goat|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000; O.D.|0.019|0.097|0.185|0.342|0.648|1.063|1.515|2.211
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|534|2702|8939|; Standard deviation|26.17|154.01|688.30|; CV(%)|4.9%|5.7%|7.7%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|578|2835|9176; Standard deviation|41.62|238.14|835.02; CV(%)|7.2%|8.4%|9.1%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 534|575|581|544|559|31.86|5.7%; Sample 2: 2702|2815|2838|2912|2817|174.65|6.2%; Sample 3: 8939|9116|8672|9284|9003|783.26|8.7%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G24-E439
• Anticoagulant: heparin, EDTA or citrate
• Principle: Human LILRA3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LILRA3 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G24-E439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LILRA3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LILRA3 amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human LILRA3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" for details. It is recommended that each human LILRA3 standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 15-20
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for LILRA3 elisa kit

This gene encodes a member of a family of immunoreceptors that are expressed predominantly in monocytes and B cells, and at lower levels in dendritic cells and natural killer cells. The encoded protein lacks the transmembrane region found in other members of this family. It acts as a soluble receptor for class I major histocompatibility complex (MHC) antigens. Alternatively spliced transcript variants encoding different isoforms have been found. This gene is located in a cluster of related genes on chromosome 19 and is polymorphic in human populations, with many individuals containing a deletion of this genomic region.

Product Categories/Family for LILRA3 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 289547633
• NCBI GeneID: 11026
• NCBI Accession #: NP_001166125.1
• NCBI GenBank Nucleotide #: NM_001172654.2
• UniProt Accession #: Q8N6C8; O15469; O15470; O75016; Q8N151; Q8N154; Q8NHJ1; Q8NHJ2; Q8NHJ3; Q8NHJ4; J3KPM2
• Molecular Weight: 49,187 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily A member 3 isoform 2
• NCBI Official Synonym Full Names: leukocyte immunoglobulin like receptor A3
• NCBI Official Symbol: LILRA3
• NCBI Official Synonym Symbols: HM31; HM43; ILT6; LIR4; CD85E; ILT-6; LIR-4
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily A member 3
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily A member 3
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRA3
• UniProt Synonym Gene Names: ILT6; LIR4; ILT-6; LIR-4

NCBI Description

This gene encodes a member of a family of immunoreceptors that are expressed predominantly in monocytes and B cells, and at lower levels in dendritic cells and natural killer cells. The encoded protein lacks the transmembrane region found in other members of this family. It acts as a soluble receptor for class I major histocompatibility complex (MHC) antigens. Alternatively spliced transcript variants encoding different isoforms have been found. This gene is located in a cluster of related genes on chromosome 19 and is polymorphic in human populations, with many individuals containing a deletion of this genomic region. [provided by RefSeq, Mar 2014]

Uniprot Description

Acts as soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.