Rabbit anti-Human, Mouse ANO5 Polyclonal Antibody | anti-ANO5 antibody

ANO5, CT (Anoctamin-5, Transmembrane protein 16E, Gnathodiaphyseal dysplasia 1 protein, TMEM16E, GDD1, ANO5)

Cat. Num. AAA628937

• Gene Names: ANO5; GDD1; LGMD2L; TMEM16E
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography followed by peptide affinity chromatography
• Synonyms: ANO5; Polyclonal Antibody; CT (Anoctamin-5; Transmembrane protein 16E; Gnathodiaphyseal dysplasia 1 protein; TMEM16E; GDD1; ANO5); Anti -ANO5; anti-ANO5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANO5. Species Crossreactivity: mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography followed by peptide affinity chromatography
• Form/Format: Supplied as a liquid in PBS containign 0.09% sodium azide

• Applicable Applications for anti-ANO5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry and Western Blot.; Dilution: ELISA: 1:1,000; Immunohistochemistry (paraffin): 1:50-1:100; Western Blot: 1:100-1:500
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa798-828 in the C-terminal region of human ANO5
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ANO5 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). ANO5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ANO5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of theANO5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ANO5 antibody

ANO5 is a member of the anoctamin family of transmembrane proteins. This protein is likely a calcium activated chloride channel.

Product Categories/Family for anti-ANO5 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 218081589
• NCBI GeneID: 203859
• NCBI Accession #: NP_001136121.1
• NCBI GenBank Nucleotide #: NM_001142649.1
• UniProt Accession #: Q75V66
• Molecular Weight: 107,188 Da
• NCBI Official Full Name: anoctamin-5 isoform b
• NCBI Official Synonym Full Names: anoctamin 5
• NCBI Official Symbol: ANO5
• NCBI Official Synonym Symbols: GDD1; LGMD2L; TMEM16E
• NCBI Protein Information: anoctamin-5; transmembrane protein 16E; integral membrane protein GDD1; gnathodiaphyseal dysplasia 1 protein; limb girdle muscular dystrophy 2L (autosomal recessive)
• UniProt Protein Name: Anoctamin-5
• Protein Family: Anoctamin
• UniProt Gene Name: ANO5
• UniProt Synonym Gene Names: GDD1; TMEM16E
• UniProt Entry Name: ANO5_HUMAN

NCBI Description

This gene encodes a member of the anoctamin family of transmembrane proteins. The encoded protein is likely a calcium activated chloride channel. Mutations in this gene have been associated with gnathodiaphyseal dysplasia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]

Uniprot Description

ANO5: May act as a calcium-activated chloride channel. Defects in ANO5 are the cause of gnathodiaphyseal dysplasia (GDD); also known as osteogenesis imperfecta with unusual skeletal lesions or gnathodiaphyseal sclerosis. GDD is a rare skeletal syndrome characterized by bone fragility, sclerosis of tubular bones, and cemento-osseous lesions of the jawbone. Patients experience frequent bone fractures caused by trivial accidents in childhood; however the fractures heal normally without bone deformity. The jaw lesions replace the tooth-bearing segments of the maxilla and mandible with fibrous connective tissues, including various amounts of cementum-like calcified mass, sometimes causing facial deformities. Patients also have a propensity for jaw infection and often suffer from purulent osteomyelitis-like symptoms, such as swelling of and pus discharge from the gums, mobility of the teeth, insufficient healing after tooth extraction and exposure of the lesions into the oral cavity. Defects in ANO5 are the cause of limb-girdle muscular dystrophy type 2L (LGMD2L). It is an autosomal recessive degenerative myopathy characterized by proximal weakness, weakness of the hip and shoulder girdles and prominent asymmetrical quadriceps femoris and biceps brachii atrophy. Defects in ANO5 are the cause of miyoshi muscular dystrophy type 3 (MMD3). It is a late-onset muscular dystrophy characterized by distal muscle weakness of the lower limbs, calf muscle discomfort and weakness, quadriceps atrophy. Muscle weakness and atrophy may be asymmetric. Belongs to the anoctamin family.

Protein type: Transporter; Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass; Channel, chloride

Chromosomal Location of Human Ortholog: 11p14.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane; intracellular; vesicle

Molecular Function: intracellular calcium activated chloride channel activity

Biological Process: chloride transport; transmembrane transport

Disease: Gnathodiaphyseal Dysplasia; Muscular Dystrophy, Limb-girdle, Type 2l; Miyoshi Muscular Dystrophy 3