Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF18A (KIF18A) Standard Curve.)
Human Kinesin-Like Protein KIF18A (KIF18A) ELISA Kit | KIF18A elisa kit
Human Kinesin-Like Protein KIF18A (KIF18A) ELISA Kit
Gene Names
KIF18A; PPP1R99; MS-KIF18A
Reactivity
Human
Synonyms
Kinesin-Like Protein KIF18A (KIF18A); Human Kinesin-Like Protein KIF18A (KIF18A) ELISA Kit; KIF18A; DKFZp434G2226; MS-KIF18A; KIF18A elisa kit
Reactivity
Human
Specificity
Human Kinesin-like protein KIF18A (KIF18A) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIF18A. No significant cross-reactivity or interference between Human KIF18A and analogues was observed.
Sequence Length
898
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF18A (KIF18A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF18A (KIF18A) Standard Curve.)
Related Product Information for KIF18A elisa kit
Description: This Human Kinesin-like protein KIF18A (KIF18A) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF18A in samples. An antibody specific for KIF18A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIF18A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF18A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF18A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: KIF18A contains a motor domain and conserved ATP- and microtubule-binding sites, and the central region is predicted to have an alpha-helical coiled-coil, followed by a C-terminal cargo-binding domain. Nuclear localization signals, protein-protein interaction motifs such as SH3, WW, AP-1, and FHA domains, and potential sites for phosphorylation and sumoylation were also identified. KIF18A shares 84% and 81% homology with the mouse and rat homologs, respectively. Indirect Immunofluorescence microscopy localized endogenous KIF18A to the nucleus, cytoplasm, and plasma membrane ruffles in preosteogenic marrow stromal cells. Punctate cytoplasmic staining of KIF18A was associated with microtubules and partial colocalization with actin.
Background: KIF18A contains a motor domain and conserved ATP- and microtubule-binding sites, and the central region is predicted to have an alpha-helical coiled-coil, followed by a C-terminal cargo-binding domain. Nuclear localization signals, protein-protein interaction motifs such as SH3, WW, AP-1, and FHA domains, and potential sites for phosphorylation and sumoylation were also identified. KIF18A shares 84% and 81% homology with the mouse and rat homologs, respectively. Indirect Immunofluorescence microscopy localized endogenous KIF18A to the nucleus, cytoplasm, and plasma membrane ruffles in preosteogenic marrow stromal cells. Punctate cytoplasmic staining of KIF18A was associated with microtubules and partial colocalization with actin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kinesin-like protein KIF18A
NCBI Official Synonym Full Names
kinesin family member 18A
NCBI Official Symbol
KIF18A
NCBI Official Synonym Symbols
PPP1R99; MS-KIF18A
NCBI Protein Information
kinesin-like protein KIF18A
UniProt Protein Name
Kinesin-like protein KIF18A
Protein Family
UniProt Gene Name
KIF18A
UniProt Synonym Gene Names
MS-KIF18A
NCBI Description
KIF18A is a member of the kinesin superfamily of microtubule-associated molecular motors (see MIM 148760) that use hydrolysis of ATP to produce force and movement along microtubules (Luboshits and Benayahu, 2005 [PubMed 15878648]).[supplied by OMIM, Aug 2008]
Uniprot Description
Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.
Research Articles on KIF18A
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Product Notes
The Human KIF18A kif18a (Catalog #AAA9715583) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715583 ELISA Kit recognizes Human KIF18A. It is sometimes possible for the material contained within the vial of "Kinesin-Like Protein KIF18A (KIF18A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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