Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF20A (KIF20A) Standard Curve.)
Human Kinesin-Like Protein KIF20A (KIF20A) ELISA Kit | KIF20A elisa kit
Human Kinesin-Like Protein KIF20A (KIF20A) ELISA Kit
Gene Names
KIF20A; MKLP2; RAB6KIFL
Reactivity
Human
Synonyms
Kinesin-Like Protein KIF20A (KIF20A); Human Kinesin-Like Protein KIF20A (KIF20A) ELISA Kit; KIF20A; FLJ21151; MKLP2; RAB6KIFL; RAB6 interacting; kinesin-like (rabkinesin6); mitotic kinesin-like protein 2; KIF20A elisa kit
Reactivity
Human
Specificity
Human Kinesin-like protein KIF20A (KIF20A) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIF20A. No significant cross-reactivity or interference between Human KIF20A and analogues was observed.
Sequence Length
890
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF20A (KIF20A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF20A (KIF20A) Standard Curve.)
Related Product Information for KIF20A elisa kit
Description: This Human Kinesin-like protein KIF20A (KIF20A) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF20A in samples. An antibody specific for KIF20A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIF20A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF20A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF20A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The RAB6KIFL gene spans approximately 8.5 kb of genomic DNA and contains 19 exons. The composite cDNA encodes a deduced 890-amino acid protein that contains a motor domain and an ATP catalytic domain, hallmarks of kinesin-like proteins, in the N terminus. The protein shares 87% sequence identity with the mouse Rab6kifl protein. In contrast to the ubiquitous expression of the mouse protein, expression of the human, approximately 3.4-kb RAB6KIFL transcript appears to be tissue specific. Northern blot analysis detected high expression in fetal tissues and in adult thymus, bone marrow, and testis, and low expression in heart, placenta, and spleen. No expression was detected in brain, lung, liver, skeletal muscle, kidney, pancreas, or peripheral leukocytes.
Background: The RAB6KIFL gene spans approximately 8.5 kb of genomic DNA and contains 19 exons. The composite cDNA encodes a deduced 890-amino acid protein that contains a motor domain and an ATP catalytic domain, hallmarks of kinesin-like proteins, in the N terminus. The protein shares 87% sequence identity with the mouse Rab6kifl protein. In contrast to the ubiquitous expression of the mouse protein, expression of the human, approximately 3.4-kb RAB6KIFL transcript appears to be tissue specific. Northern blot analysis detected high expression in fetal tissues and in adult thymus, bone marrow, and testis, and low expression in heart, placenta, and spleen. No expression was detected in brain, lung, liver, skeletal muscle, kidney, pancreas, or peripheral leukocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kinesin-like protein KIF20A
NCBI Official Synonym Full Names
kinesin family member 20A
NCBI Official Symbol
KIF20A
NCBI Official Synonym Symbols
MKLP2; RAB6KIFL
NCBI Protein Information
kinesin-like protein KIF20A
UniProt Protein Name
Kinesin-like protein KIF20A
Protein Family
UniProt Gene Name
KIF20A
UniProt Synonym Gene Names
MKLP2; RAB6KIFL; MKlp2
UniProt Entry Name
KI20A_HUMAN
Uniprot Description
KIF20A: Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. Belongs to the kinesin-like protein family.
Protein type: Microtubule-binding; Motor
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm; Golgi apparatus; kinesin complex; microtubule; spindle; midbody
Molecular Function: protein binding; transporter activity; microtubule binding; ATPase activity; microtubule motor activity; protein kinase binding; ATP binding
Biological Process: vesicle-mediated transport; protein transport; cytokinesis after mitosis; cell separation during cytokinesis; metabolic process; cytokinesis; mitotic cell cycle; microtubule-based movement; microtubule bundle formation
Research Articles on KIF20A
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Product Notes
The Human KIF20A kif20a (Catalog #AAA9715581) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715581 ELISA Kit recognizes Human KIF20A. It is sometimes possible for the material contained within the vial of "Kinesin-Like Protein KIF20A (KIF20A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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