Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF14 (KIF14) Standard Curve.)
Human Kinesin-Like Protein KIF14 (KIF14) ELISA Kit | KIF14 elisa kit
Human Kinesin-Like Protein KIF14 (KIF14) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF14 (KIF14) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin-like protein KIF14 (KIF14) Standard Curve.)
Background: KIF14 is a member of the kinesin superfamily of microtubule-associated motors that play important roles in intracellular transport and cell division.KIF14 protein contains an internal motor domain with a conserved catalytic core, an N-type conserved neck region, a forkhead-associated (FHA) domain, and 4 coiled-coil domains. Immunofluorescence microscopy localized KIF14 throughout the cell cycle in 293T cells and HeLa cells. During interphase, KIF14 localized to the cytoplasm and was redistributed to the nucleus upon entry into mitosis. From prophase to metaphase, KIF14 localized at developing spindle poles and associated microtubules, and during anaphase, KIF14 accumulated at the spindle midzone, where it concentrated during telophase. KIF14 localized to the contractile ring in cells ready to undergo abscission.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Uniprot Description
KIF14: Plays an essential role in cytokinesis. Belongs to the kinesin-like protein family.
Protein type: Microtubule-binding; Motor
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: microtubule; kinesin complex; membrane; cytoplasm; plasma membrane; spindle midzone; midbody; nucleus
Molecular Function: tubulin binding; protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; protein kinase binding; ATP binding; PDZ domain binding
Biological Process: positive regulation of cytokinesis; regulation of Rap protein signal transduction; regulation of cell adhesion; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; cerebellar granular layer structural organization; regulation of myelination; cell proliferation in forebrain; olfactory bulb development; hippocampus development; cytoskeleton-dependent intracellular transport; negative regulation of integrin activation; regulation of cell migration; mitotic metaphase plate congression; microtubule-based movement; cerebellar cortex development; establishment of protein localization; regulation of G2/M transition of mitotic cell cycle; regulation of neuron apoptosis; positive regulation of cell proliferation; activation of protein kinase activity; microtubule depolymerization; negative regulation of neuron apoptosis; cerebral cortex development; regulation of cell growth; cerebellar Purkinje cell layer structural organization; negative regulation of apoptosis
Disease: Meckel Syndrome 12
Research Articles on KIF14
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Product Notes
The Human KIF14 kif14 (Catalog #AAA9715584) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715584 ELISA Kit recognizes Human KIF14. It is sometimes possible for the material contained within the vial of "Kinesin-Like Protein KIF14 (KIF14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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