Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Kinesin-like protein KIF2B (KIF2B) Standard Curve.)
Rat Kinesin-Like Protein KIF2B (KIF2B) ELISA Kit | KIF2B elisa kit
Rat Kinesin-Like Protein KIF2B (KIF2B) ELISA Kit
Reactivity
Rat
Synonyms
Kinesin-Like Protein KIF2B (KIF2B); Rat Kinesin-Like Protein KIF2B (KIF2B) ELISA Kit; KIF2B; FLJ53902; kinesin protein; KIF2B elisa kit
Reactivity
Rat
Specificity
Rat Kinesin-like protein KIF2B (KIF2B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KIF2B. No significant cross-reactivity or interference between Rat KIF2B and analogues was observed.
Sequence Length
664
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Kinesin-like protein KIF2B (KIF2B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Kinesin-like protein KIF2B (KIF2B) Standard Curve.)
Related Product Information for KIF2B elisa kit
Description: This Rat Kinesin-like protein KIF2B (KIF2B) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF2B in samples. An antibody specific for KIF2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIF2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Kinesins are microtubule-based motor proteins involved in the transport of organelles in eukaryotic cells. They The predicted 963-amino acid protein has 63% sequence identity to the Drosophila KHC. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunofluorescence studies showed that KNS1 expressed in CV-1 cells had both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules but not vimentin (VIM) intermediate filaments; the KNS1 N- and C-terminal domains, but not the alpha-helical rod domain, also colocalized with microtubules.
Background: Kinesins are microtubule-based motor proteins involved in the transport of organelles in eukaryotic cells. They The predicted 963-amino acid protein has 63% sequence identity to the Drosophila KHC. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunofluorescence studies showed that KNS1 expressed in CV-1 cells had both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules but not vimentin (VIM) intermediate filaments; the KNS1 N- and C-terminal domains, but not the alpha-helical rod domain, also colocalized with microtubules.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kinesin-like protein KIF2B
NCBI Official Synonym Full Names
kinesin family member 2B
NCBI Official Symbol
Kif2b
NCBI Protein Information
kinesin-like protein KIF2B
UniProt Protein Name
Kinesin-like protein KIF2B
Protein Family
UniProt Gene Name
Kif2b
UniProt Entry Name
KIF2B_RAT
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Product Notes
The Rat KIF2B kif2b (Catalog #AAA9712510) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712510 ELISA Kit recognizes Rat KIF2B. It is sometimes possible for the material contained within the vial of "Kinesin-Like Protein KIF2B (KIF2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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