Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Interferon alpha-21 (IFNA21) Standard Curve.)
Human Interferon alpha-21 (IFNA21) ELISA Kit | IFNA21 elisa kit
Human Interferon alpha-21 (IFNA21) ELISA Kit
Gene Names
IFNA21; LeIF F; leIF-F; IFN-alphaI
Reactivity
Human
Synonyms
Interferon alpha-21 (IFNA21); Human Interferon alpha-21 (IFNA21) ELISA Kit; IFNA21; MGC126687; MGC126689; leukocyte interferon protein; IFNA21 elisa kit
Reactivity
Human
Specificity
Human Interferon alpha-21 (IFNA21) ELISA Kit has high sensitivity and excellent specificity for detection of Human IFNA21. No significant cross-reactivity or interference between Human IFNA21 and analogues was observed.
Sequence Length
189
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Interferon alpha-21 (IFNA21) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Interferon alpha-21 (IFNA21) Standard Curve.)
Related Product Information for IFNA21 elisa kit
Description: This Human Interferon alpha-21 (IFNA21) ELISA Kit employs a two-site sandwich ELISA to quantitate IFNA21 in samples. An antibody specific for IFNA21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNA21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNA21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNA21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The IFNA21 gene was positioned in the cluster of interferon genes on 9p22 by deletion mapping. Interferons (IFNs) are proteins made and released by lymphocytes in response to the presence of pathogens—such as viruses, bacteria, or parasites—or tumor cells. IFNs belong to the large class of glycoproteins known as cytokines. Although they are named after their ability to "interfere" with viral replication within host cells, IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection.
Background: The IFNA21 gene was positioned in the cluster of interferon genes on 9p22 by deletion mapping. Interferons (IFNs) are proteins made and released by lymphocytes in response to the presence of pathogens—such as viruses, bacteria, or parasites—or tumor cells. IFNs belong to the large class of glycoproteins known as cytokines. Although they are named after their ability to "interfere" with viral replication within host cells, IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interferon alpha-21
NCBI Official Synonym Full Names
interferon alpha 21
NCBI Official Symbol
IFNA21
NCBI Official Synonym Symbols
LeIF F; leIF-F; IFN-alphaI
NCBI Protein Information
interferon alpha-21
UniProt Protein Name
Interferon alpha-21
Protein Family
UniProt Gene Name
IFNA21
UniProt Synonym Gene Names
IFN-alpha-21; LeIF F
NCBI Description
This gene is a member of the alpha interferon gene cluster on the short arm of chromosome 9. Interferons are cytokines produced in response to viral infection that mediate the immune response and interfere with viral replication. The encoded protein is a type I interferon and may play a specific role in the antiviral response to rubella virus. [provided by RefSeq, Sep 2011]
Uniprot Description
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Research Articles on IFNA21
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Product Notes
The Human IFNA21 ifna21 (Catalog #AAA9715907) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715907 ELISA Kit recognizes Human IFNA21. It is sometimes possible for the material contained within the vial of "Interferon alpha-21 (IFNA21), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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