Human Interferon Alpha 21 (IFNa21) ELISA Kit | IFNa21 elisa kit

Human Interferon Alpha 21 (IFNa21) ELISA Kit

Cat. Num. AAA2021447

• Gene Names: IFNA21; LeIF F; leIF-F; IFN-alphaI
• Reactivity: Human
• Synonyms: Interferon Alpha 21 (IFNa21); Human Interferon Alpha 21 (IFNa21) ELISA Kit; LeIF F; Interferon alpha-F; IFNa21 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Alpha 21 (IFNa21).; No significant cross-reactivity or interference between Interferon Alpha 21 (IFNa21) and analogues was observed.
• Sequence Length: 189

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 21 (IFNa21) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 21 (IFNa21) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFNa21 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 21 (IFNa21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha 21 (IFNa21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha 21 (IFNa21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Alpha 21 (IFNa21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IFNa21 elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 169791013
• NCBI GeneID: 3452
• NCBI Accession #: NP_002166.2
• NCBI GenBank Nucleotide #: NM_002175.2
• UniProt Accession #: P01568; Q14608; Q5VWD1; Q7M4Q4
• Molecular Weight: 21,741 Da
• NCBI Official Full Name: interferon alpha-21
• NCBI Official Synonym Full Names: interferon, alpha 21
• NCBI Official Symbol: IFNA21
• NCBI Official Synonym Symbols: LeIF F; leIF-F; IFN-alphaI
• NCBI Protein Information: interferon alpha-21
• UniProt Protein Name: Interferon alpha-21
• Protein Family: Interferon
• UniProt Gene Name: IFNA21
• UniProt Synonym Gene Names: IFN-alpha-21; LeIF F
• UniProt Entry Name: IFN21_HUMAN

NCBI Description

This gene is a member of the alpha interferon gene cluster on the short arm of chromosome 9. Interferons are cytokines produced in response to viral infection that mediate the immune response and interfere with viral replication. The encoded protein is a type I interferon and may play a specific role in the antiviral response to rubella virus. [provided by RefSeq, Sep 2011]

Uniprot Description

IFNA21: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; cytokine and chemokine mediated signaling pathway; B cell differentiation; regulation of MHC class I biosynthetic process; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein