Monkey Interferon alpha-21 ELISA Kit | IFNA21 elisa kit

Monkey Interferon alpha-21 ELISA Kit

Cat. Num. AAA747138

• Gene Names: IFNA21; LeIF F; leIF-F; IFN-alphaI
• Reactivity: Monkey
• Synonyms: Interferon alpha-21; Monkey Interferon alpha-21 ELISA Kit; IFNA21 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This IFNa21 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IFNa21. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFNA21 elisa kit

Principle of the assay: IFNa21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNa21 antibody and an IFNa21-HRP conjugate. The assay sample and buffer are incubated together with IFNa21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNa21 concentration since IFNa21 from samples and IFNa21-HRP conjugate compete for the anti-IFNa21 antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNa21 from the sample, fewer sites are left to bind IFNa21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNa21 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IFNA21 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 169791013
• NCBI GeneID: 3452
• NCBI Accession #: NP_002166.2
• NCBI GenBank Nucleotide #: NM_002175.2
• Molecular Weight: 21,741 Da
• NCBI Official Full Name: interferon alpha-21
• NCBI Official Synonym Full Names: interferon, alpha 21
• NCBI Official Symbol: IFNA21
• NCBI Official Synonym Symbols: LeIF F; leIF-F; IFN-alphaI
• NCBI Protein Information: interferon alpha-21; IFN-alpha-21; interferon alpha-F; leukocyte interferon protein
• UniProt Protein Name: Interferon alpha-21
• Protein Family: Interferon
• UniProt Gene Name: IFNA21
• UniProt Synonym Gene Names: IFN-alpha-21; LeIF F
• UniProt Entry Name: IFN21_HUMAN

NCBI Description

This gene is a member of the alpha interferon gene cluster on the short arm of chromosome 9. Interferons are cytokines produced in response to viral infection that mediate the immune response and interfere with viral replication. The encoded protein is a type I interferon and may play a specific role in the antiviral response to rubella virus. [provided by RefSeq, Sep 2011]

Uniprot Description

IFNA21: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein