Human IFNA21 (IFN alpha 21) ELISA Kit | IFNA21 elisa kit

Human IFNA21 (IFN alpha 21) ELISA Kit

Cat. Num. AAA7606848

• Gene Names: IFNA21; LeIF F; leIF-F; IFN-alphaI
• Reactivity: Human
• Synonyms: IFNA21 (IFN alpha 21); Human IFNA21 (IFN alpha 21) ELISA Kit; IFNA21 elisa kit

• Reactivity: Human
• Sequence Length: 189

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFNA21 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IFNA21 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IFNA21 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IFNA21 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IFNA21 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 169791013
• NCBI GeneID: 3452
• NCBI Accession #: NP_002166.2
• NCBI GenBank Nucleotide #: NM_002175.2
• UniProt Accession #: P01568; Q14608; Q5VWD1; Q7M4Q4
• Molecular Weight: 21,741 Da
• NCBI Official Full Name: interferon alpha-21
• NCBI Official Synonym Full Names: interferon alpha 21
• NCBI Official Symbol: IFNA21
• NCBI Official Synonym Symbols: LeIF F; leIF-F; IFN-alphaI
• NCBI Protein Information: interferon alpha-21
• UniProt Protein Name: Interferon alpha-21
• Protein Family: Interferon
• UniProt Gene Name: IFNA21
• UniProt Synonym Gene Names: IFN-alpha-21; LeIF F

NCBI Description

This gene is a member of the alpha interferon gene cluster on the short arm of chromosome 9. Interferons are cytokines produced in response to viral infection that mediate the immune response and interfere with viral replication. The encoded protein is a type I interferon and may play a specific role in the antiviral response to rubella virus. [provided by RefSeq, Sep 2011]

Uniprot Description

IFNA21: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p21.3

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding

Biological Process: adaptive immune response; B cell differentiation; B cell proliferation; blood coagulation; cytokine and chemokine mediated signaling pathway; humoral immune response; innate immune response; natural killer cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to exogenous dsRNA; T cell activation during immune response