Human CHKA (Choline kinase alpha) ELISA Kit | CHKA elisa kit

Human CHKA (Choline kinase alpha) ELISA Kit

Cat. Num. AAA7608159

• Gene Names: CHKA; CK; EK; CHK; CKI
• Reactivity: Human
• Synonyms: CHKA (Choline kinase alpha); Human CHKA (Choline kinase alpha) ELISA Kit; Choline kinase alpha; CHKA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHKA. No significant cross-reactivity or interference between CHKA and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHKA elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 226694197
• NCBI GeneID: 1119
• UniProt Accession #: P35790; Q8NE29
• Molecular Weight: 457
• NCBI Official Full Name: Choline kinase alpha
• NCBI Official Synonym Full Names: choline kinase alpha
• NCBI Official Symbol: CHKA
• NCBI Official Synonym Symbols: CK; EK; CHK; CKI
• NCBI Protein Information: choline kinase alpha; CHETK-alpha; ethanolamine kinase
• UniProt Protein Name: Choline kinase alpha
• Protein Family: Choline kinase
• UniProt Gene Name: CHKA
• UniProt Synonym Gene Names: CHK; CKI; CK; EK
• UniProt Entry Name: CHKA_HUMAN

NCBI Description

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CHKA: Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; EC 2.7.1.82; Kinase, other; EC 2.7.1.32

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytosol

Molecular Function: choline binding; signal transducer activity; protein homodimerization activity; cholinesterase activity; drug binding; ethanolamine kinase activity; choline kinase activity; ATP binding

Biological Process: choline metabolic process; CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process; signal transduction; lipid transport; phosphorylation