Typical Testing Data/Standard Curve (for reference only)
Human Huntingtin Interacting Protein 12 (HIP12) ELISA Kit | HIP12 elisa kit
Human Huntingtin Interacting Protein 12 (HIP12) ELISA Kit
Gene Names
HIP1R; HIP3; HIP12; ILWEQ
Reactivity
Human
Synonyms
Huntingtin Interacting Protein 12 (HIP12); Human Huntingtin Interacting Protein 12 (HIP12) ELISA Kit; HIP12; HIP3; ILWEQ; Huntingtin Interacting Protein 12; HIP12 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity for detection of HIF2a. 100% cross-reactivity of HIF2a was observed among mouse and rat.
Sequence Length
890
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.057ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HIF2a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HIF2a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HIP12 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HIF2a in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to HIF2a. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HIF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIF2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HIF2a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to HIF2a. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HIF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIF2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HIF2a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for HIP12 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,288 Da
NCBI Official Full Name
huntingtin interacting protein 1 related, isoform CRA_a
NCBI Official Synonym Full Names
huntingtin interacting protein 1 related
NCBI Official Symbol
HIP1R
NCBI Official Synonym Symbols
HIP3; HIP12; ILWEQ
NCBI Protein Information
huntingtin-interacting protein 1-related protein
UniProt Protein Name
Huntingtin-interacting protein 1-related protein
UniProt Gene Name
HIP1R
UniProt Synonym Gene Names
HIP12; KIAA0655; HIP1-related protein; HIP-12
Uniprot Description
Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
Research Articles on HIP12
Similar Products
Product Notes
The Human HIP12 hip1r (Catalog #AAA2104888) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104888 ELISA Kit recognizes Human HIP12. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the HIP12 hip1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Huntingtin Interacting Protein 12 (HIP12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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