Mouse HIP1R Polyclonal Antibody | anti-HIP1R antibody

HIP1R (Huntingtin-interacting Protein 1-related Protein, HIP1-related Protein, Huntingtin-interacting Protein 12, HIP-12, HIP12, KIAA0655)

Cat. Num. AAA646428

• Gene Names: HIP1R; HIP3; HIP12; ILWEQ
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HIP1R; Polyclonal Antibody; HIP1R (Huntingtin-interacting Protein 1-related Protein; HIP1-related Protein; Huntingtin-interacting Protein 12; HIP-12; HIP12; KIAA0655); Anti -HIP1R (Huntingtin-interacting Protein 1-related Protein; anti-HIP1R antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HIP1R. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MNSIKNVPARVLSRRPGHSLEAEREQFDKTQAISISKAINTQEAPVKEKHARRIILGTHHEKGAFTFWSYAIGLPLPSSSILSWKFCHVLHKVLRDGHPNVLHDCQRYRSNIREIGDLWGHLHDRYGQLVNVYTKLLLTKISFHLKHPQFPAGLEVTDEVLEKAAGTDVNNIFQLTVEMFDYMDCELKLSESVFRQLNTAIAVSQMSSGQCRLAPLIQVIQDCSHLYHYTVKLLFKLHSCLPADTLQGHRDRFHEQFHSLRNFFRRASDMLYFKRLIQIPRLPEGPPNFLRASALAEHIKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPNGSVKDDRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQGRLAERESQEQGLRQRLLDEQFAVLRGAAAEAAGILQDAVSKLDDPLHLRCTSSPDYLVSRAQEALDAVSTLEEGHAQYLTSLADASALVAALTRFSHLAADTIINGGATSHLAPTDPADRLIDTCRECGARALELMGQLQDQQALRHMQASLVRTPLQGILQLGQELKPKSLDVRQEELGAVVDKEMAATSAAIEDAVRRIEDMMNQARHASSGVKLEVNERILNSCTDLMKAIRLLVTTSTSLQKEIVESGRGAATQQEFYAKNSRWTEGLISASKAVGWGATQLVEAADKVVLHTGKYEELIVCSHEIAASTAQLVAASKVKANKHSPHLSRLQECSRTVNERAANVVASTKSGQEQIEDRDTMDFSGLSLIKLKKQEMETQVRVLELEKTLEAERMRLGELRKQHYVLAGASGSPGEEVAIRPSTAPRSVTTKKPPLAQKPSVAPRQDHQLDKKDGIYPAQLVNY

• Applicable Applications for anti-HIP1R antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human HIP1R, aa1-1068 (NP_003950.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to HIP1R on HeLa cell. [antibody concentration 10ug/ml])

Western Blot (WB)

(Western Blot analysis of HIP1R expression in transfected 293T cell line by HIP1R polyclonal antibody. Lane 1: HIP1R transfected lysate (117.48kD). Lane 2: Non-transfected lysate.)

Western Blot (WB)

(HIP1R polyclonal antibody. Western Blot analysis of HIP1R expression in NIH/3T3.)

Western Blot (WB)

(HIP1R polyclonal antibody. Western Blot analysis of HIP1R expression in PC-12.)

Related Product Information for anti-HIP1R antibody

Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.

Product Categories/Family for anti-HIP1R antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 48762942
• NCBI GeneID: 9026
• NCBI Accession #: NP_003950.1
• NCBI GenBank Nucleotide #: NM_003959.1
• UniProt Accession #: O75146; Q9UED9; A6NHQ6
• Molecular Weight: 119,388 Da
• NCBI Official Full Name: huntingtin-interacting protein 1-related protein
• NCBI Official Synonym Full Names: huntingtin interacting protein 1 related
• NCBI Official Symbol: HIP1R
• NCBI Official Synonym Symbols: HIP3; HIP12; ILWEQ
• NCBI Protein Information: huntingtin-interacting protein 1-related protein; HIP-12; HIP1-related protein; huntingtin interacting protein 12; huntingtin-interacting protein 12
• UniProt Protein Name: Huntingtin-interacting protein 1-related protein
• Protein Family: Huntingtin-interacting protein 1-related protein
• UniProt Gene Name: HIP1R
• UniProt Synonym Gene Names: HIP12; KIAA0655; HIP1-related protein; HIP-12
• UniProt Entry Name: HIP1R_HUMAN

Uniprot Description

Function: Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. Ref.5 Ref.6

Subunit structure: Interacts with actin. Does not interact with huntingtin

By similarity. Interacts with CLTB and HIP1. Homodimer. Homodimerization promotes actin binding. Ref.5 Ref.7 Ref.11

Subcellular location: Cytoplasm › perinuclear region. Endomembrane system. Cytoplasmic vesicle › clathrin-coated vesicle membrane. Note: Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region. Ref.5 Ref.6

Tissue specificity: Brain, heart, kidney, pancreas, and liver, but not in lung or placenta.

Domain: Binds F-actin via the talin-like I/LWEQ domain.

Sequence similarities: Belongs to the SLA2 family.Contains 1 ENTH (epsin N-terminal homology) domain.Contains 1 I/LWEQ domain.

Sequence caution: The sequence BAA31630.1 differs from that shown. Reason: Erroneous initiation.