Mouse Small Ubiquitin Related Modifier Protein 1 (SUMO1) ELISA Kit | SUMO1 elisa kit

Mouse Small Ubiquitin Related Modifier Protein 1 (SUMO1) High Sensitive ELISA Kit

Cat. Num. AAA2708115

• Gene Names: SUMO1; ARABIDOPSIS THALIANA SMALL UBIQUITIN-LIKE MODIFIER 1; ATSUMO1; F10M23.180; F10M23_180; small ubiquitin-like modifier 1; SMALL UBIQUITIN-LIKE MODIFIER 1; SUM1; SUMO 1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Small Ubiquitin Related Modifier Protein 1 (SUMO1); Mouse Small Ubiquitin Related Modifier Protein 1 (SUMO1) High Sensitive ELISA Kit; Small Ubiquitin Related Modifier Protein 1; GMP1; OFC10; PIC1; SMT3; SMT3C; SMT3H3; SUMO-1; UBL1; GAP-modifying protein 1; Ubiquitin-homology domain protein PIC1; SUMO1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1).; No significant cross-reactivity or interference between High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1) and analogues was observed.
• Sequence Length: 100

• Applicable Applications for SUMO1 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Detection Range: 78-5000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 27pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Small Ubiquitin Related Modifier Protein 1 (SUMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 15236885
• NCBI GeneID: 828791
• NCBI GenBank Nucleotide #: NP_194414.1
• UniProt Accession #: P55852
• NCBI Official Full Name: small ubiquitin-like modifier 1
• NCBI Official Symbol: SUMO1
• NCBI Official Synonym Symbols: ARABIDOPSIS THALIANA SMALL UBIQUITIN-LIKE MODIFIER 1; ATSUMO1; F10M23.180; F10M23_180; small ubiquitin-like modifier 1; SMALL UBIQUITIN-LIKE MODIFIER 1; SUM1; SUMO 1
• NCBI Protein Information: small ubiquitin-like modifier 1
• UniProt Protein Name: Small ubiquitin-related modifier 1
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO1
• UniProt Synonym Gene Names: SMT3; SUM1; AtSUMO1

NCBI Description

Encodes a small ubiquitin-like modifier (SUMO) polypeptide that becomes covalently attached to various intracellular protein targets, much like ubiquitination, leading to post-translational modification of those targets.

Uniprot Description

Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Required for the massive protein sumoylation in the nucleus induced by heat shock and controlled by SIZ1. Involved in the regulation of the heat stress transcription factor HSFA2 in acquired thermotolerance.