Mouse Lymphotoxin Beta (LTb) CLIA Kit | LTb clia kit

Mouse Lymphotoxin Beta (LTb) CLIA Kit

Cat. Num. AAA2001216

• Gene Names: LTB; p33; TNFC; TNFSF3
• Reactivity: Mouse
• Synonyms: Lymphotoxin Beta (LTb); Mouse Lymphotoxin Beta (LTb) CLIA Kit; LT-B; p33; TNFC; TNF-C; TNFSF3; Tumor necrosis factor ligand superfamily member 3; Tumor Necrosis Factor C; LTb clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lymphotoxin Beta (LTb).; No significant cross-reactivity or interference between Lymphotoxin Beta (LTb) and analogues was observed.
• Sequence Length: 244

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: < 0.58pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphotoxin Beta (LTb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphotoxin Beta (LTb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTb clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Lymphotoxin Beta (LTb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lymphotoxin Beta (LTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lymphotoxin Beta (LTb) level in the sample or standard.;

Product Categories/Family for LTb clia kit

Cytokine; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4337113
• NCBI GeneID: 4050
• UniProt Accession #: Q06643; P78370; Q52LU8; Q99761
• Molecular Weight: 7,780 Da
• NCBI Official Full Name: lymphotoxin beta
• NCBI Official Synonym Full Names: lymphotoxin beta (TNF superfamily, member 3)
• NCBI Official Symbol: LTB
• NCBI Official Synonym Symbols: p33; TNFC; TNFSF3
• NCBI Protein Information: lymphotoxin-beta
• UniProt Protein Name: Lymphotoxin-beta
• Protein Family: Lymphotoxin
• UniProt Gene Name: LTB
• UniProt Synonym Gene Names: TNFC; TNFSF3; LT-beta; TNF-C
• UniProt Entry Name: TNFC_HUMAN

NCBI Description

Lymphotoxin beta is a type II membrane protein of the TNF family. It anchors lymphotoxin-alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the lymphotoxin-alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin-beta receptor. The minor complex is lymphotoxin-alpha 2/beta 1. LTB is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin-beta isoform b is unable to complex with lymphotoxin-alpha suggesting a function for lymphotoxin-beta which is independent of lympyhotoxin-alpha. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

LTB: Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: skin development; positive regulation of interleukin-12 biosynthetic process; cell-cell signaling; gene expression; immune response; signal transduction; lymph node development