Human Alpha-globin transcription factor CP2 ELISA Kit | TFCP2 elisa kit

Human Alpha-globin transcription factor CP2 ELISA Kit

Cat. Num. AAA763778

• Gene Names: TFCP2; LSF; SEF; LBP1C; LSF1D; TFCP2C
• Reactivity: Human
• Synonyms: Alpha-globin transcription factor CP2; Human Alpha-globin transcription factor CP2 ELISA Kit; TFCP2/Alpha-globin transcription factor CP2/SAA3 enhancer factor/Transcription factor LSF/LSF/SEF/LBP1C/LSF1D/TFCP2C; TFCP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TFCP2. No significant cross-reactivity or interference between TFCP2 and analogues was observed.
• Sequence Length: 450

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFCP2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 291219876
• NCBI GeneID: 7024
• NCBI Accession #: NP_001166924.1
• NCBI GenBank Nucleotide #: NM_001173453.1
• Molecular Weight: 57,185 Da
• NCBI Official Full Name: alpha-globin transcription factor CP2 isoform 3
• NCBI Official Synonym Full Names: transcription factor CP2
• NCBI Official Symbol: TFCP2
• NCBI Official Synonym Symbols: LSF; SEF; LBP1C; LSF1D; TFCP2C
• NCBI Protein Information: alpha-globin transcription factor CP2
• UniProt Protein Name: Alpha-globin transcription factor CP2
• Protein Family: Alpha-globin transcription factor
• UniProt Gene Name: TFCP2
• UniProt Synonym Gene Names: LSF; SEF
• UniProt Entry Name: TFCP2_HUMAN

NCBI Description

This gene encodes a transcription factor that binds the alpha-globin promoter and activates transcription of the alpha-globin gene. The encoded protein regulates erythroid gene expression, plays a role in the transcriptional switch of globin gene promoters, and it activates many other cellular and viral gene promoters. The gene product interacts with certain inflammatory response factors, and polymorphisms of this gene may be involved in the pathogenesis of Alzheimer's disease. [provided by RefSeq, Mar 2010]

Uniprot Description

TFCP2: Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter. Belongs to the grh/CP2 family. CP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleus

Molecular Function: DNA binding; protein binding; protein C-terminus binding; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent