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Western Blot (WB) (Western Blot (WB) analysis of VGLL2 polyclonal antibody at 1:500 dilutionLane1:The Heart tissue lysate of Rat (40ug) Lane2:The Heart tissue lysate of Mouse (40ug) Lane3:Hela whole cell lysate (40ug))

Rabbit VGLL2 Polyclonal Antibody | anti-VGLL2 antibody

VGLL2 Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
VGLL2; Polyclonal Antibody; VGLL2 Polyclonal Antibody; Transcription cofactor vestigial-like protein 2; Vgl-2; Protein VITO1; VITO1; anti-VGLL2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
VGLL2 polyclonal antibody detects endogenous levels of VGLL2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-VGLL2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 34-79 of Human VGLL2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of VGLL2 polyclonal antibody at 1:500 dilutionLane1:The Heart tissue lysate of Rat (40ug) Lane2:The Heart tissue lysate of Mouse (40ug) Lane3:Hela whole cell lysate (40ug))

Western Blot (WB) (Western Blot (WB) analysis of VGLL2 polyclonal antibody at 1:500 dilutionLane1:The Heart tissue lysate of Rat (40ug) Lane2:The Heart tissue lysate of Mouse (40ug) Lane3:Hela whole cell lysate (40ug))
Related Product Information for anti-VGLL2 antibody
Vgl-2 (Transcription cofactor vestigial-like protein 2), also known as VITO-1, is a 317 amino acid protein that contains a domain through which it interacts with TEF-1, a protein that plays a role in controlling the expression of numerous genes. Specific to skeletal muscle, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle. During muscle differentiation, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus.Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), which is a marker of terminal muscle differentiation. This evidence suggests that Vgl-2 is essential for muscle gene expression. There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events.

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
~ 33kDa

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Product Notes

The VGLL2 (Catalog #AAA3007380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VGLL2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VGLL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the VGLL2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VGLL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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