Rabbit anti-Human, Mouse CDH3 Polyclonal Antibody | anti-CDH3 antibody

CDH3, NT (CDH3, CDHP, Cadherin-3, Placental cadherin) (AP)

Cat. Num. AAA6284182

• Gene Names: CDH3; CDHP; HJMD; PCAD
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDH3; Polyclonal Antibody; NT (CDH3; CDHP; Cadherin-3; Placental cadherin) (AP); Placental cadherin; anti-CDH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CDH3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CDH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121~150 amino acids from the N-terminal region of human CDH3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDH3 Antibody (N-term) in NIH-3T3 cell line lysates (35ug/lane). CDH3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CDH3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using CDH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDH3 antibody

CDH3 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in the gene encoding CDH3 have been associated with congential hypotrichosis with juvenile macular dystrophy.

Product Categories/Family for anti-CDH3 antibody

Antibodies; Cadherin

References

Indelman, M., Clin. Exp. Dermatol. 32 (2), 191-196 (2007). Paredes, J., Clin. Cancer Res. 11 (16), 5869-5877 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 14589891
• NCBI GeneID: 1001
• NCBI Accession #: NP_001784.2
• NCBI GenBank Nucleotide #: NM_001793.4
• UniProt Accession #: P22223; Q05DI6; B2R6F4
• Molecular Weight: 91,418 Da
• NCBI Official Full Name: cadherin-3 preproprotein
• NCBI Official Synonym Full Names: cadherin 3, type 1, P-cadherin (placental)
• NCBI Official Symbol: CDH3
• NCBI Official Synonym Symbols: CDHP; HJMD; PCAD
• NCBI Protein Information: cadherin-3; calcium-dependent adhesion protein, placental
• UniProt Protein Name: Cadherin-3
• UniProt Gene Name: CDH3
• UniProt Synonym Gene Names: CDHP; P-cadherin
• UniProt Entry Name: CADH3_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell-cell adherens junction; cytoplasm; integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: response to drug; positive regulation of insulin-like growth factor receptor signaling pathway; intercellular junction assembly and maintenance; wound healing; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; retinal homeostasis; negative regulation of transforming growth factor-beta2 production; hair cycle process; cell-cell adhesion; positive regulation of monophenol monooxygenase activity; negative regulation of catagen; visual perception; keratinization; cell adhesion; homophilic cell adhesion

Disease: Ectodermal Dysplasia, Ectrodactyly, And Macular Dystrophy Syndrome; Hypotrichosis, Congenital, With Juvenile Macular Dystrophy