Human Gelsolin (GS) ELISA Kit | GS elisa kit

Human Gelsolin (GS) ELISA Kit

Cat. Num. AAA2701701

• Reactivity: Human
• Synonyms: Gelsolin (GS); Human Gelsolin (GS) ELISA Kit; GSN; AGEL; ADF; Brevin; Gelsolin Amyloidosis; Finnish Type; Actin-depolymerizing factor; GS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Gelsolin (GS).; No significant cross-reactivity or interference between Gelsolin (GS) and analogues was observed.
• Sequence Length: 780

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 62.5-4000pg/mL
• Sensitivity: < 28.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gelsolin (GS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gelsolin (GS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gelsolin (GS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gelsolin (GS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gelsolin (GS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gelsolin (GS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51854227
• NCBI GeneID: 296654
• NCBI Accession #: NP_001004080.1
• NCBI GenBank Nucleotide #: NM_001004080.1
• UniProt Accession #: Q68FP1
• Molecular Weight: 80,929 Da
• NCBI Official Full Name: gelsolin
• NCBI Official Synonym Full Names: gelsolin
• NCBI Official Symbol: Gsn
• NCBI Protein Information: gelsolin
• UniProt Protein Name: Gelsolin
• UniProt Gene Name: Gsn
• UniProt Synonym Gene Names: ADF

NCBI Description

plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]

Uniprot Description

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p11

Cellular Component: actin cap; actin cytoskeleton; cortical actin cytoskeleton; cytoplasm; cytosol; extracellular region; extracellular space; focal adhesion; lamellipodium; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; protein complex; ruffle; sarcoplasm

Molecular Function: actin binding; actin filament binding; calcium ion binding; myosin II binding; protein domain specific binding

Biological Process: actin filament capping; actin filament polymerization; actin filament severing; actin nucleation; actin polymerization or depolymerization; aging; apoptosis; barbed-end actin filament capping; extracellular matrix disassembly; negative regulation of virion penetration into host cell; oligodendrocyte development; phagocytosis, engulfment; phosphoinositide-mediated signaling; positive regulation of actin nucleation; protein destabilization; regulation of cell adhesion; response to ethanol; response to folic acid; sequestering of actin monomers; striated muscle atrophy; tissue regeneration; vesicle-mediated transport; wound healing