Human Fibroblast Growth Factor Receptor 1 (FGFR1) ELISA Kit | FGFR1 elisa kit

Human Fibroblast Growth Factor Receptor 1 (FGFR1) ELISA Kit

Cat. Num. AAA2019897

• Reactivity: Human
• Synonyms: Fibroblast Growth Factor Receptor 1 (FGFR1); Human Fibroblast Growth Factor Receptor 1 (FGFR1) ELISA Kit; CD331; FLT2; KAL2; CEK; FLG; BFGFR; N-SAM; Basic Fibroblast Growth Factor Receptor 1; Fms-Related Tyrosine Kinase-2; Pfeiffer Syndrome; FGFR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGFR1. No significant cross-reactivity or interference between FGFR1 and analogues was observed.
• Sequence Length: 822

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGFR1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGFR1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for FGFR1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGFR1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FGFR1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FGFR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGFR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FGFR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FGFR1 elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity

NCBI Description

proportion of transmembrane and soluble extracellular isoforms may modulate fibroblast growth factor signaling; plays a role in regulation of neuronal cell proliferation [RGD, Feb 2006]

Uniprot Description

FGFR1 iso2: a receptor tyrosine kinase of the highly-conserved fibroblast growth factor receptor (FGFR). Binds both acidic and basic fibroblast growth factors and is involved in limb induction. Point mutations cause Pfeffer syndrome (finger and toe malformations and other skeletal errors) and dominant Kallmann syndrome 2. Stem cell leukemia lymphoma syndrome (SCLL) may be caused by a t(8;13)(p12;q12) translocation that fuses a zinc finger gene, ZNF198, to FGFR1. Various myeloproliferative disorders have been linked to translocations that fuse FGFR1 to FOP, FIM, CEP1 or the atypical kinase, Bcr. Inhibitor: SU5402. 20 isoforms of the human protein produced by alternative splicing have been described.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Membrane protein, integral; Protein kinase, TK; TK group; FGFR family

Cellular Component: cytoplasmic membrane-bound vesicle; integral to membrane; plasma membrane; cytosol; nucleus; receptor complex

Molecular Function: heparin binding; identical protein binding; protein binding; protein homodimerization activity; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; protein complex binding; cell adhesion molecule binding; glycoprotein binding; ATP binding

Biological Process: paraxial mesoderm development; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; cell maturation; middle ear morphogenesis; negative regulation of transcription from RNA polymerase II promoter; orbitofrontal cortex development; positive regulation of MAP kinase activity; sensory perception of sound; positive regulation of MAPKKK cascade; ureteric bud development; induction of an organ; salivary gland morphogenesis; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; midbrain development; chondrocyte differentiation; negative regulation of osteoblast differentiation; angiogenesis; neurite development; central nervous system neuron development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation; inner ear morphogenesis; motogenic signaling involved in postnatal olfactory bulb interneuron migration; fibroblast growth factor receptor signaling pathway; in utero embryonic development; stem cell maintenance; outer ear morphogenesis; positive regulation of cell cycle; regulation of cell proliferation; neuroblast division in the ventricular zone; mesenchymal cell differentiation; generation of neurons; regulation of gene expression; blood vessel morphogenesis; auditory receptor cell development; positive regulation of transcription from RNA polymerase II promoter; brain development; regulation of sensory perception of pain; positive regulation of neuron differentiation; regulation of lateral mesodermal cell fate specification; lung development