Typical Testing Data/Standard Curve (for reference only)
Rat Corticotropin Releasing Hormone ELISA Kit | Crh elisa kit
Rat Corticotropin Releasing Hormone ELISA Kit
Reactivity
Rat
Synonyms
Corticotropin Releasing Hormone; Rat Corticotropin Releasing Hormone ELISA Kit; Crh (Corticotropin Releasing Hormone)/Corticoliberin/Corticotropin-releasing factor/CRF/Corticotropin-releasing hormone; Crh elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Cr. No significant cross-reactivity or interference between Cr and analogues was observed.
Sequence Length
122
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Competitive
Detection Range
1.25-80nmol/ml
Sensitivity
<0.75nmol/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cr were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cr were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Crh elisa kit
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Cr. During the reaction, Cr in the sample or standard competes with a fixed amount of Cr on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Cr. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Cr in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,547 Da
NCBI Official Full Name
corticotropin releasing hormone, partial
NCBI Official Symbol
CRH
NCBI Protein Information
corticoliberin
UniProt Protein Name
Corticoliberin
Protein Family
UniProt Gene Name
CRH
UniProt Synonym Gene Names
CRF
UniProt Entry Name
CRF_CANLF
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Product Notes
The Rat Crh crh (Catalog #AAA764613) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA764613 ELISA Kit recognizes Rat Crh. It is sometimes possible for the material contained within the vial of "Corticotropin Releasing Hormone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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