Typical Testing Data/Standard Curve (for reference only)
Human GS (Gelsolin) CLIA Kit | GS clia kit
Human GS (Gelsolin) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GS. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GS. You can calculate the concentration of GS in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]
Uniprot Description
Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.
Protein type: Nuclear receptor co-regulator; Actin-binding; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted
Cellular Component: extracellular space; cortical actin cytoskeleton; protein complex; focal adhesion; extracellular region; cytosol; actin cytoskeleton; ruffle; perinuclear region of cytoplasm; lamellipodium; cytoplasm; plasma membrane; podosome; actin cap; nucleus; sarcoplasm; myelin sheath
Molecular Function: protein domain specific binding; calcium ion binding; actin binding; myosin II binding
Biological Process: regulation of cell adhesion; actin filament severing; phosphoinositide-mediated signaling; protein destabilization; wound healing; actin filament capping; apoptosis; negative regulation of virion penetration into host cell; actin nucleation; vesicle-mediated transport; extracellular matrix disassembly; response to ethanol; striated muscle atrophy; actin filament polymerization; tissue regeneration; sequestering of actin monomers; positive regulation of actin nucleation; response to folic acid; actin polymerization and/or depolymerization; oligodendrocyte development; phagocytosis, engulfment; barbed-end actin filament capping; aging
Research Articles on GS
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Product Notes
The Human GS gsn (Catalog #AAA2531650) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531650 CLIA Kit recognizes Human GS. It is sometimes possible for the material contained within the vial of "GS (Gelsolin), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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