Human ECM1 ELISA Kit | ECM1 elisa kit

Human ECM1 (Extracellular Matrix Protein 1) ELISA Kit

Cat. Num. AAA2505556

• Gene Names: ECM1; URBWD
• Reactivity: Human
• Synonyms: ECM1; Human ECM1 (Extracellular Matrix Protein 1) ELISA Kit; ECM1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human ECM1 in samples. No significant cross-reactivity or interference between Human ECM1 and analogues was observed.
• Sequence Length: 567

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ECM1 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ECM1 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ECM1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ECM1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ECM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ECM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ECM1. You can calculate the concentration of Human ECM1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 322302700
• NCBI GeneID: 1893
• NCBI Accession #: NP_001189787.1
• NCBI GenBank Nucleotide #: NM_001202858.1
• UniProt Accession #: Q16610; O43266; Q5T5G4; Q5T5G5; Q5T5G6; Q8IZ60; A8K8S0; B4DW49; B4DY60
• Molecular Weight: 63,563 Da
• NCBI Official Full Name: extracellular matrix protein 1 isoform 3
• NCBI Official Synonym Full Names: extracellular matrix protein 1
• NCBI Official Symbol: ECM1
• NCBI Official Synonym Symbols: URBWD
• NCBI Protein Information: extracellular matrix protein 1; secretory component p85
• UniProt Protein Name: Extracellular matrix protein 1
• Protein Family: Extracellular matrix protein
• UniProt Gene Name: ECM1
• UniProt Entry Name: ECM1_HUMAN

NCBI Description

This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space

Molecular Function: protein C-terminus binding; signal transducer activity; protein binding; enzyme binding; protease binding; interleukin-2 receptor binding; laminin binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of peptidase activity; signal transduction; regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; positive regulation of angiogenesis; biomineral formation; positive regulation of endothelial cell proliferation; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; regulation of T-helper 2 type immune response; inflammatory response

Disease: Lipoid Proteinosis Of Urbach And Wiethe