Human IL-1RAcP/IL-1R3/IL1RAP ELISA Kit | IL1RAP elisa kit

Human IL-1RAcP/IL-1R3/IL1RAP PicoKine ELISA Kit

Cat. Num. AAA1752565

• Gene Names: IL1RAP; IL1R3; C3orf13; IL-1RAcP
• Reactivity: Human
• Synonyms: IL-1RAcP/IL-1R3/IL1RAP; Human IL-1RAcP/IL-1R3/IL1RAP PicoKine ELISA Kit; Human IL-1RAcP/IL-1R3/IL1RAP; interleukin 1 receptor accessory protein; Interleukin-1 receptor accessory protein; IL-1 receptor accessory protein; IL-1RAcP; Interleukin-1 receptor 3; IL-1R-3; IL-1R3; IL1RAP; C3orf13; IL1R3; IL1RAP elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL1RAP
• Sequence Length: 356

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL1RAP elisa kit

Principle of the Assay: The Picokine™ Human IL1RAP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL1RAP with a 96-well strip plate that is pre-coated with antibody specific for IL1RAP. The detection antibody is a biotinylated antibody specific for IL1RAP. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human IL1RAP with immunogen: Expression system for standard: NSO, Immunogen sequence: S21-T367. The kit is analytically validated with ready to use reagents. To measure Human IL1RAP, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human IL1RAP in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL1RAP in the sample.!!Background/Introduction: Interleukin-1 receptor accessory protein is a protein that in humans is encoded by the IL1RAP gene. It is mapped to 3q28. This gene encodes a component of the interleukin 1 receptor complex, which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in membrane-bound and soluble isoforms differing in their C-terminus. The ratio of soluble to membranebound forms increases during acute-phase induction or stress.

NCBI and Uniprot Product Information

• NCBI GI #: 268840341
• NCBI GeneID: 3556
• NCBI Accession #: NP_001161402.1
• NCBI GenBank Nucleotide #: NP_001161402.1
• UniProt Accession #: Q9NPH3
• NCBI Official Full Name: interleukin-1 receptor accessory protein isoform 2
• NCBI Official Synonym Full Names: interleukin 1 receptor accessory protein
• NCBI Official Symbol: IL1RAP
• NCBI Official Synonym Symbols: IL1R3; C3orf13; IL-1RAcP
• NCBI Protein Information: interleukin-1 receptor accessory protein
• UniProt Protein Name: Interleukin-1 receptor accessory protein
• Protein Family: Interleukin-1 receptor accessory protein
• UniProt Gene Name: IL1RAP
• UniProt Synonym Gene Names: C3orf13; IL1R3; IL-1 receptor accessory protein; IL-1RAcP; IL-1R-3; IL-1R3
• UniProt Entry Name: IL1AP_HUMAN

NCBI Description

This gene encodes a component of the interleukin 1 receptor complex, which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in membrane-bound and soluble isoforms differing in their C-terminus. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. [provided by RefSeq, Jul 2018]

Uniprot Description

IL1RAP: Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: membrane; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: signal transducer activity; interleukin-1 receptor activity

Biological Process: interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; innate immune response; immune response; protein complex assembly; inflammatory response