Typical Testing Data/Standard Curve (for reference only)
Human Estrone ELISA Kit
Estrone ELISA
Reactivity
Human
Synonyms
Estrone; Estrone ELISA; Estrone elisa kit
Reactivity
Human
Assay Type
Quantitative Competitive
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Estrone elisa kit
Intended Uses: For the direct quantitative determination of Estrone in human serum by an enzyme immunoassay. For in vitro use only.
Principle of the Assay: The Estrone ELISA is a competitive immunoassay. Competition occurs between estrone present in standards, controls and patient samples and an enzyme-labelled antigen (conjugate) for a limited number of anti-estrone antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the enzyme substrate is added and 20 minutes later the enzymatic reaction is terminated by addition of stopping solution. The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentration of estrone in the sample. A calibrator curve is plotted with a provided set of calibrators to directly calculate the concentration of estrone in patient samples and controls.
Background/Introduction: Estrone is a steroid like estriol and estradiol, belonging to the class of estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract in order to prepare it for the fertilized ovum.
Principle of the Assay: The Estrone ELISA is a competitive immunoassay. Competition occurs between estrone present in standards, controls and patient samples and an enzyme-labelled antigen (conjugate) for a limited number of anti-estrone antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the enzyme substrate is added and 20 minutes later the enzymatic reaction is terminated by addition of stopping solution. The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentration of estrone in the sample. A calibrator curve is plotted with a provided set of calibrators to directly calculate the concentration of estrone in patient samples and controls.
Background/Introduction: Estrone is a steroid like estriol and estradiol, belonging to the class of estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract in order to prepare it for the fertilized ovum.
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Product Notes
The Human Estrone (Catalog #AAA495628) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495628 ELISA Kit recognizes Human Estrone. It is sometimes possible for the material contained within the vial of "Estrone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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