Typical Testing Data/Standard Curve (for reference only)
Human CYFRA ELISA Kit | CYFRA elisa kit
CYFRA 21-1 ELISA
Reactivity
Human
Synonyms
CYFRA; CYFRA 21-1 ELISA; CYFRA elisa kit
Reactivity
Human
Specificity
The CYFRA 21-1 EIA assay mean assay specificity is 100 +/- 15%. Recovery studies were performed to compare sera containing the following compounds at the indicated concentrations with control sera. The CLSI guideline EP7-A (24) was used to design the interference experiments. The following substances and concentrations were tested and found not to interfere with the test.
Assay Type
Competitive
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CYFRA elisa kit
Intended Uses: The CYFRA 21-1 EIA kit is intended for the determination of soluble cytokeratin 19 fragments in human serum. The assay is to be used as an aid in monitoring disease progression during the course of disease and treatment in lung cancer patients. Serial testing for patient CYFRA 21-1 assay values should be used in conjunction with other clinical methods used for monitoring lung cancer.
Principle of the Assay: The CYFRA 21-1 EIA is a solid phase, non-competitive immunoassay based on two monoclonal antibodies (derived from mice) directed against two separate antigenic determinants of soluble fragments of cytokeratin 19 (7-8). Calibrators, controls and patient samples are incubated together with biotinylated Anti-CYFRA 21-1 MAb and horseradish peroxidase (HRP) labeled Anti-CYFRA 21-1 MAb in streptavidin coated microstrips. After washing, buffered Substrate/Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetramethylbenzidine) is added to each well and the enzyme reaction is allowed to proceed. During the enzyme reaction a blue color will develop if antigen is present. The intensity of the color development is proportional to the amount of CYFRA 21-1 present in the samples. The color intensity is determined in a microplate spectrophotometer at 620 nm (or optionally at 405 nm after addition of Stop Solution). Calibration curves are constructed for each assay by plotting absorbance value versus the concentration for each calibrator. The CYFRA 21-1 concentrations of patient samples are then read from the calibration curve.
Principle of the Assay: The CYFRA 21-1 EIA is a solid phase, non-competitive immunoassay based on two monoclonal antibodies (derived from mice) directed against two separate antigenic determinants of soluble fragments of cytokeratin 19 (7-8). Calibrators, controls and patient samples are incubated together with biotinylated Anti-CYFRA 21-1 MAb and horseradish peroxidase (HRP) labeled Anti-CYFRA 21-1 MAb in streptavidin coated microstrips. After washing, buffered Substrate/Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetramethylbenzidine) is added to each well and the enzyme reaction is allowed to proceed. During the enzyme reaction a blue color will develop if antigen is present. The intensity of the color development is proportional to the amount of CYFRA 21-1 present in the samples. The color intensity is determined in a microplate spectrophotometer at 620 nm (or optionally at 405 nm after addition of Stop Solution). Calibration curves are constructed for each assay by plotting absorbance value versus the concentration for each calibrator. The CYFRA 21-1 concentrations of patient samples are then read from the calibration curve.
Product Categories/Family for CYFRA elisa kit
Similar Products
Product Notes
The Human CYFRA (Catalog #AAA494749-211-10) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494749-211-10 ELISA Kit recognizes Human CYFRA. It is sometimes possible for the material contained within the vial of "CYFRA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
