Typical Testing Data/Standard Curve (for reference only)
Human Testosterone ELISA Kit
Testosterone Free ELISA
Reactivity
Human
Synonyms
Testosterone; Testosterone Free ELISA; Testosterone elisa kit
Reactivity
Human
Specificity
The following compounds were tested for cross-reactivity with the Direct Free Testosterone ELISA kit with testosterone crossreacting at 100%.
Samples
Human serum
Assay Type
Quantitative Competitive
Sensitivity
0.018 pg/mL
Intra-assay Precision
Five samples were assayed 24 times each on the same calibrator curve. The results (in pg/mL) are tabulated below
Inter-assay Precision
Three samples were assayed twenty times in duplicate over a period of greater than ten days. The results (in pg/mL) are tabulated below
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Testosterone elisa kit
Intended Uses: For the direct quantitative determination of Free Testosterone by an enzyme immunoassay in human serum.
Principle of the Assay: The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of free testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of free testosterone in patient samples and controls can be directly read. The free testosterone kit utilizes a highly specifi c rabbit antitestosterone polyclonal antibody at a low binding capacity (Keq x concentration) to keep minimum disturbances of the testosterone-protein equilibrium. The other components in the test system are also optimized in order to not alter the original free testosterone concentration.
Principle of the Assay: The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of free testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of free testosterone in patient samples and controls can be directly read. The free testosterone kit utilizes a highly specifi c rabbit antitestosterone polyclonal antibody at a low binding capacity (Keq x concentration) to keep minimum disturbances of the testosterone-protein equilibrium. The other components in the test system are also optimized in order to not alter the original free testosterone concentration.
Product Categories/Family for Testosterone elisa kit
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Product Notes
The Human Testosterone (Catalog #AAA494226) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494226 ELISA Kit recognizes Human Testosterone. It is sometimes possible for the material contained within the vial of "Testosterone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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