Human Endothelin Receptor A (ETRA) ELISA Kit | ETRA elisa kit

Human Endothelin Receptor A (ETRA) ELISA Kit

Cat. Num. AAA453554

• Gene Names: EDNRA; ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR
• Reactivity: Human
• Synonyms: Endothelin Receptor A (ETRA); Human Endothelin Receptor A (ETRA) ELISA Kit; EDNRA; ETA; ETR-A; Endothelin A receptor; Endothelin-1 receptor; ETRA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ETRA. No significant cross-reactivity or interference between ETRA and analogues was observed.
• Sequence Length: 318

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ETRA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ETRA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ETRA elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ETRA in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ETRA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ETRA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ETRA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ETRA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 260593650
• NCBI GeneID: 1909
• NCBI Accession #: NP_001159527.1
• NCBI GenBank Nucleotide #: NM_001166055.1
• UniProt Accession #: P25101; O43441; Q16432; Q16433; Q8TBH2; B2R723; B4E2V6; B7Z9G6; D3DP03; E7ER36; AAB24923
• Molecular Weight: 23,803 Da
• NCBI Official Full Name: endothelin-1 receptor isoform b
• NCBI Official Synonym Full Names: endothelin receptor type A
• NCBI Official Symbol: EDNRA
• NCBI Official Synonym Symbols: ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR
• NCBI Protein Information: endothelin-1 receptor
• UniProt Protein Name: Endothelin-1 receptor
• Protein Family: Electron transport regulator
• UniProt Gene Name: EDNRA
• UniProt Synonym Gene Names: ETA; ETRA; ET-A; ETA-R; hET-AR

NCBI Description

This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.