Typical Testing Data/Standard Curve (for reference only)
Mouse Endothelin Receptor A ELISA Kit | ETRA elisa kit
Mouse Endothelin Receptor A ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ETRA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ETRA antibody and an ETRA-HRP conjugate. The assay sample and buffer are incubated together with ETRA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ETRA concentration since ETRA from samples and ETRA-HRP conjugate compete for the anti-ETRA antibody binding site. Since the number of sites is limited, as more sites are occupied by ETRA from the sample, fewer sites are left to bind ETRA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ETRA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Uniprot Description
EDNRA: Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 4q31.22
Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; lipid raft
Molecular Function: endothelin receptor activity; protein binding; phosphoinositide phospholipase C activity
Biological Process: positive regulation of odontogenesis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); neural crest cell development; metabolic process; heart development; smooth muscle cell proliferation; response to morphine; response to lipopolysaccharide; glucose transport; signal transduction; sensory perception of pain; enteric nervous system development; regulation of epithelial cell proliferation; Rho protein signal transduction; histamine secretion; negative regulation of cAMP biosynthetic process; elevation of cytosolic calcium ion concentration; smooth muscle contraction; fibroblast proliferation; regulation of blood pressure; positive regulation of cell proliferation; protein kinase C activation; artery smooth muscle contraction; aging; penile erection; vasoconstriction; in utero embryonic development; adenylate cyclase activation; glomerular filtration; respiratory gaseous exchange; patterning of blood vessels; cell proliferation; G-protein coupled receptor protein signaling pathway; phospholipase C activation; maternal process involved in parturition; response to hypoxia; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of apoptosis; positive regulation of inflammatory response
Disease: Mandibulofacial Dysostosis With Alopecia; Migraine With Or Without Aura, Susceptibility To, 1
Research Articles on ETRA
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Product Notes
The Mouse ETRA ednra (Catalog #AAA723986) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723986 ELISA Kit recognizes Mouse ETRA. It is sometimes possible for the material contained within the vial of "Endothelin Receptor A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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