Human Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) ELISA Kit | NBC4 elisa kit

Human Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) ELISA Kit

Cat. Num. AAA2023174

• Gene Names: SLC4A5; NBC4
• Reactivity: Human
• Synonyms: Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4); Human Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) ELISA Kit; SLC4A5; NBCe2; Solute Carrier Family 4 Member 5; Sodium Bicarbonate Cotransporter; NBC4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4).; No significant cross-reactivity or interference between Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) and analogues was observed.
• Sequence Length: 1,067

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NBC4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NBC4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 125987596
• NCBI GeneID: 57835
• NCBI Accession #: NP_067019.3
• NCBI GenBank Nucleotide #: NM_021196.3
• UniProt Accession #: Q9BY07; Q32MA7; Q59EQ9; Q8WXD3; Q8WXD7; Q96DS7; Q96DS8; Q9HBU5
• Molecular Weight: 118,125 Da
• NCBI Official Full Name: electrogenic sodium bicarbonate cotransporter 4 isoform a
• NCBI Official Synonym Full Names: solute carrier family 4 (sodium bicarbonate cotransporter), member 5
• NCBI Official Symbol: SLC4A5
• NCBI Official Synonym Symbols: NBC4
• NCBI Protein Information: electrogenic sodium bicarbonate cotransporter 4
• UniProt Protein Name: Electrogenic sodium bicarbonate cotransporter 4
• UniProt Gene Name: SLC4A5
• UniProt Entry Name: S4A5_HUMAN

NCBI Description

This gene encodes a member of the sodium bicarbonate cotransporter (NBC) family, part of the bicarbonate transporter superfamily. Sodium bicarbonate cotransporters are involved in intracellular pH regulation and electroneural or electrogenic sodium bicarbonate transport. This protein is thought to be an integral membrane protein. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC4A5: Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na(+):HCO3(-) stoichiometry of 2:1. May have a housekeeping function in regulating the pH of tissues in which it is expressed. May play a role in mediating Na(+):HCO3(-) cotransport in hepatocytes and intrahepatic cholangiocytes. Also may be important in protecting the renal paranchyma from alterations in urine pH. Belongs to the anion exchanger (TC 2.A.31) family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: integral to plasma membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: inorganic anion exchanger activity; sodium:bicarbonate symporter activity

Biological Process: renal system process; cerebrospinal fluid secretion; retina development in camera-type eye; regulation of gene expression; bicarbonate transport; regulation of intracellular pH; regulation of systemic arterial blood pressure; epithelial cell development; ion transport; transmembrane transport; mitochondrion distribution