Rabbit anti-Human, Mouse IFT57 Polyclonal Antibody | anti-IFT57 antibody

IFT57 (DERP8, ESRRBL1, HIPPI, Intraflagellar Transport Protein 57 Homolog, Dermal Papilla-derived Protein 8, Estrogen-related Receptor beta-like Protein 1, HIP1-interacting Protein, MHS4R2, FLJ10147) (FITC)

Cat. Num. AAA6382423

• Gene Names: IFT57; HIPPI; MHS4R2; ESRRBL1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IFT57; Polyclonal Antibody; IFT57 (DERP8; ESRRBL1; HIPPI; Intraflagellar Transport Protein 57 Homolog; Dermal Papilla-derived Protein 8; Estrogen-related Receptor beta-like Protein 1; HIP1-interacting Protein; MHS4R2; FLJ10147) (FITC); anti-IFT57 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IFT57. Species Crossreactivity: Mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-IFT57 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human IFT57, aa1-429 (NP_060480.1).
• Immunogen Sequence: MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALKETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVMEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATVIPEPATGFY
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IFT57 rabbit polyclonal antibody. Western Blot analysis of IFT57 expression in mouse liver.)

Western Blot (WB)

(Western Blot analysis of IFT57 expression in transfected 293T cell line by IFT57 polyclonal antibody. Lane 1: IFT57 transfected lysate (49.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-IFT57 antibody

IFT57 is required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

Product Categories/Family for anti-IFT57 antibody

Antibodies; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 8922256
• NCBI GeneID: 55081
• NCBI Accession #: NP_060480.1
• NCBI GenBank Nucleotide #: NM_018010.2
• UniProt Accession #: Q9NWB7; Q96DA9
• Molecular Weight: 49,108 Da
• NCBI Official Full Name: intraflagellar transport protein 57 homolog
• NCBI Official Synonym Full Names: intraflagellar transport 57
• NCBI Official Symbol: IFT57
• NCBI Official Synonym Symbols: HIPPI; MHS4R2; ESRRBL1
• NCBI Protein Information: intraflagellar transport protein 57 homolog; HIP1 protein interactor; HIP1-interacting protein; dermal papilla-derived protein 8; intraflagellar transport 57 homolog; estrogen-related receptor beta like 1; estrogen-related receptor beta-like protein 1; hu
• UniProt Protein Name: Intraflagellar transport protein 57 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT57
• UniProt Synonym Gene Names: DERP8; ESRRBL1; HIPPI
• UniProt Entry Name: IFT57_HUMAN

Uniprot Description

IFT57: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro- apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear. Belongs to the IFT57 family.

Chromosomal Location of Human Ortholog: 3q13.13

Cellular Component: Golgi apparatus; centrosome; photoreceptor connecting cilium; cilium

Molecular Function: protein binding; DNA binding

Biological Process: regulation of apoptosis; caspase activation; smoothened signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; organelle organization and biogenesis; neural tube closure; intraflagellar transport; cilium biogenesis; heart looping; negative regulation of epithelial cell proliferation