Dot Blot (DB)
(Dot blot analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 5C11.1. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
Mouse 4-Hydroxy-2-hexenal Monoclonal Antibody | anti-4-HHE antibody
4-Hydroxy-2-hexenal Antibody, Clone 5C11.1: FITC
Reactivity
Species Independent
Applications
Western Blot, ELISA
Purity
Protein G Purified
Synonyms
4-Hydroxy-2-hexenal; Monoclonal Antibody; 4-Hydroxy-2-hexenal Antibody; Clone 5C11.1: FITC; Mouse Anti-Mouse 4-Hydroxy-2-hexenal Monoclonal IgG1; 4-Hydroxy-2-hexenal Antibody: FITC; 4-Hydroxy-2-hexenal (4-HHE) Antibody; 4-HHE Antibody; MG Antibody; MG-modified protein Antibody; 4-Hydroxy-2-hexenal-modified; 4-hydroxy Hexenal Antibody; HHE Antibody; anti-4-HHE antibody
Host
Mouse
Reactivity
Species Independent
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5C11.1
Specificity
Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-4-HHE antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000
ELISA: 1:1000
ELISA: 1:1000
Target
4-Hydroxy-2-hexenal
Immunogen
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
Conjugate
FITC
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.
Dot Blot (DB)
(Dot blot analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 5C11.1. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
Dot Blot (DB)
(Dot blot analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 5C11.1. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
Product Categories/Family for anti-4-HHE antibody
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Product Notes
The 4-HHE (Catalog #AAA809036) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 4-Hydroxy-2-hexenal Antibody, Clone 5C11.1: FITC reacts with Species Independent and may cross-react with other species as described in the data sheet. AAA Biotech's 4-Hydroxy-2-hexenal can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the 4-HHE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4-Hydroxy-2-hexenal, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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