Mouse RhoA,B,C Monoclonal Antibody | anti-RHOA antibody

RhoA,B,C (GDP/GTP Binding Protein) (Biotin)

Cat. Num. AAA6242918

• Gene Names: RHOA; ARHA; ARH12; RHO12; RHOH12
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: RhoA; B; C; Monoclonal Antibody; C (GDP/GTP Binding Protein) (Biotin); anti-RHOA antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4F5
• Specificity: Recognizes human Rho (-A, -B, -C), Mr 22kD. Species Crossreactivity: rat and mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RHOA antibody: Western Blot (WB)
• Immunogen: Recombinant human RhoA containing amino acids 1-155.
• Conjugate: Biotin
• Preparation and Storage: Store at -20 degree C.

Testing Data

(IB: HL-60 cell lysate was probed with anti-Rho (-A, -B, -C) (3ug/ml).)

Product Categories/Family for anti-RHOA antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Arthur, W.T., et al., J. Biol. Chem. 277: 42964-42972, 2002. 2. DerMardirossian, C. and Bokoch, G.M., Drug News Perspect., 14: 389-395, 2001. 3. Symons, M. and Rusk, N., Curr. Biol., 13: 409-418, 2003. 4. Raftopoulou, M. and Hall, A., Dev. Biol., 265: 23-32, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 10835049
• NCBI GeneID: 387
• NCBI Accession #: NP_001655
• NCBI GenBank Nucleotide #: NM_001664.3
• UniProt Accession #: P61586; P06749; Q53HM4; Q5U024; Q9UDJ0; Q9UEJ4
• Molecular Weight: 25.7 kDa (227aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: transforming protein RhoA isoform 1
• NCBI Official Synonym Full Names: ras homolog family member A
• NCBI Official Symbol: RHOA
• NCBI Official Synonym Symbols: ARHA; ARH12; RHO12; RHOH12
• NCBI Protein Information: transforming protein RhoA
• UniProt Protein Name: Transforming protein RhoA
• Protein Family: GTP-binding protein
• UniProt Gene Name: RHOA
• UniProt Synonym Gene Names: ARH12; ARHA; RHO12; h12
• UniProt Entry Name: RHOA_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Uniprot Description

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; cell cortex; midbody; cytosol; cell junction; cleavage furrow

Molecular Function: GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; transforming growth factor beta receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; apical junction assembly; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation