Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human WD repeat-containing protein mio (MIOS) Standard Curve.)
Human WD Repeat-Containing Protein MIO (MIOS) ELISA Kit | MIOS elisa kit
Human WD Repeat-Containing Protein MIO (MIOS) ELISA Kit
Gene Names
MIOS; MIO; Sea4; Yulink
Reactivity
Human
Synonyms
WD Repeat-Containing Protein MIO (MIOS); Human WD Repeat-Containing Protein MIO (MIOS) ELISA Kit; MIOS; FLJ20323; H_DJ1159O04.1; OTTHUMP00000201671; WUGSC:H_DJ1159O04.1; missing oocyte; meiosis regulator; homolog; MIOS elisa kit
Reactivity
Human
Specificity
Human WD repeat-containing protein mio (MIOS) ELISA Kit has high sensitivity and excellent specificity for detection of Human MIOS. No significant cross-reactivity or interference between Human MIOS and analogues was observed.
Sequence Length
875
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human WD repeat-containing protein mio (MIOS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human WD repeat-containing protein mio (MIOS) Standard Curve.)
Related Product Information for MIOS elisa kit
Description: This Human WD repeat-containing protein mio (MIOS) ELISA Kit employs a two-site sandwich ELISA to quantitate MIOS in samples. An antibody specific for MIOS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIOS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIOS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIOS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MIOS (Meiosis Regulator For Oocyte Development) is a Protein Coding gene. Among its related pathways are mTOR signaling pathway (KEGG) and PI3K/ Akt Signaling. As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and GATSL3.
Background: MIOS (Meiosis Regulator For Oocyte Development) is a Protein Coding gene. Among its related pathways are mTOR signaling pathway (KEGG) and PI3K/ Akt Signaling. As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and GATSL3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
GATOR complex protein MIOS isoform 1
NCBI Official Synonym Full Names
meiosis regulator for oocyte development
NCBI Official Symbol
MIOS
NCBI Official Synonym Symbols
MIO; Sea4; Yulink
NCBI Protein Information
GATOR complex protein MIOS
UniProt Protein Name
WD repeat-containing protein mio
Protein Family
UniProt Gene Name
MIOS
UniProt Entry Name
MIO_HUMAN
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Product Notes
The Human MIOS mios (Catalog #AAA9715274) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715274 ELISA Kit recognizes Human MIOS. It is sometimes possible for the material contained within the vial of "WD Repeat-Containing Protein MIO (MIOS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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