Rabbit anti-Human Zyxin Polyclonal Antibody | anti-ZYX antibody

Zyxin (ZYX, ZYX Protein, ESP 2, ESP2, HED 2, HED2, Zyxin 2) (PE)

Cat. Num. AAA6399392

• Gene Names: ZYX; ESP-2; HED-2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Zyxin; Polyclonal Antibody; Zyxin (ZYX; ZYX Protein; ESP 2; ESP2; HED 2; HED2; Zyxin 2) (PE); anti-ZYX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZYX.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ZYX antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ZYX, aa1-572 (NP_001010972.1)
• Immunogen Sequence: MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX polyclonal antibody. Lane 1: ZYX transfected lysate (61.3kD). Lane 2: Non-transfected lysate. Protocol Download)

Related Product Information for anti-ZYX antibody

ZYX gene is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains. This protein localizes to focal adhesion sites and along actin stress fibers. Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid (LPA)-dependent manner and it regulates LPA-induced cell migration. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Product Categories/Family for anti-ZYX antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 58530845
• NCBI GeneID: 7791
• NCBI Accession #: NP_001010972.1
• NCBI GenBank Nucleotide #: NM_001010972.1
• UniProt Accession #: Q15942; Q6I9S4; A4D2G6
• Molecular Weight: 61,277 Da
• NCBI Official Full Name: zyxin
• NCBI Official Synonym Full Names: zyxin
• NCBI Official Symbol: ZYX
• NCBI Official Synonym Symbols: ESP-2; HED-2
• NCBI Protein Information: zyxin; zyxin-2
• UniProt Protein Name: Zyxin
• Protein Family: Zyxin
• UniProt Gene Name: ZYX
• UniProt Entry Name: ZYX_HUMAN

NCBI Description

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

Zyxin: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Belongs to the zyxin/ajuba family.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: focal adhesion; cell-cell adherens junction; integral to plasma membrane; cytoplasm; stress fiber; plasma membrane; nucleus

Molecular Function: protein binding; zinc ion binding

Biological Process: integrin-mediated signaling pathway; viral reproduction; cell-cell signaling; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; regulation of inflammatory response; stress fiber formation; signal transduction; cell adhesion