Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Claudin-8 (CLDN8) Standard Curve.)
Human Claudin-8 (CLDN8) ELISA Kit | CLDN8 elisa kit
Human Claudin-8 (CLDN8) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Claudin-8 (CLDN8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Claudin-8 (CLDN8) Standard Curve.)
Background: Claudins, such as CLDN8, are components of epithelial cell tight junctions. Tight junctions regulate movement of solutes and ions through the paracellular space and prevent mixing of proteins and lipids in the outer leaflet of the apical and basolateral plasma membrane domains. Cldn8 expression reduced paracellular permeability to monovalent inorganic and organic cations and to divalent cations, but not to anions or neutral substrates. The results were consistent with a model in which CLDN2 encodes a highly cation-permeable channel, whereas CLDN8 acts primarily as a cation barrier. Cldn8 was expressed primarily at the tight junction along the entire aldosterone-sensitive distal nephron and in the late segments of the thin descending limbs of long-looped nephrons.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein plays important roles in the paracellular cation barrier of the distal renal tubule, and in the paracellular barrier to prevent sodium back-leakage in distal colon. Differential expression of this gene has been observed in colorectal carcinoma and renal cell tumors, and along with claudin-7, is an immunohistochemical marker for the differential diagnosis of chromophobe renal cell carcinoma and renal oncocytoma.[provided by RefSeq, May 2010]
Uniprot Description
Claudin-8: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.
Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: tight junction; endoplasmic reticulum; basolateral plasma membrane; plasma membrane; integral to membrane
Molecular Function: identical protein binding; structural molecule activity
Biological Process: calcium-independent cell-cell adhesion
Research Articles on CLDN8
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Product Notes
The Human CLDN8 cldn8 (Catalog #AAA9715942) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715942 ELISA Kit recognizes Human CLDN8. It is sometimes possible for the material contained within the vial of "Claudin-8 (CLDN8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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