Rabbit anti-Human CLDN8 Polyclonal Antibody | anti-CLDN8 antibody

CLDN8, ID (CLDN8, Claudin-8) (AP)

Cat. Num. AAA6286536

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLDN8; Polyclonal Antibody; ID (CLDN8; Claudin-8) (AP); Claudin-8; anti-CLDN8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CLDN8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion of amino acids within aa140-166 from the Center region of human CLDN8, conjugated to KLH.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CLDN8 Antibody (Center) in 293 cell line lysates (35ug/lane). CLDN8 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(CLDN8 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CLDN8 antibody

CLDN8 is components of epithelial cell tight junctions. Tight junctions regulate movement of solutes and ions through the paracellular space and prevent mixing of proteins and lipids in the outer leaflet of the apical and basolateral plasma membrane domains

Product Categories/Family for anti-CLDN8 antibody

Antibodies; Claudin

References

Kim, S.S., et.al., Histopathology 54 (5), 633-635 (2009). Osunkoya, A.O., et.al, Hum. Pathol. 40 (2), 206-210 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 40788011
• NCBI GeneID: 9073
• NCBI Accession #: NP_955360.1
• NCBI GenBank Nucleotide #: NM_199328.2
• UniProt Accession #: P56748; Q53EX7; D3DSE3
• Molecular Weight: 24,845 Da
• NCBI Official Full Name: claudin-8
• NCBI Official Synonym Full Names: claudin 8
• NCBI Official Symbol: CLDN8
• NCBI Protein Information: claudin-8
• UniProt Protein Name: Claudin-8
• Protein Family: Claudin
• UniProt Gene Name: CLDN8
• UniProt Entry Name: CLD8_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein plays important roles in the paracellular cation barrier of the distal renal tubule, and in the paracellular barrier to prevent sodium back-leakage in distal colon. Differential expression of this gene has been observed in colorectal carcinoma and renal cell tumors, and along with claudin-7, is an immunohistochemical marker for the differential diagnosis of chromophobe renal cell carcinoma and renal oncocytoma.[provided by RefSeq, May 2010]

Uniprot Description

Claudin-8: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: tight junction; endoplasmic reticulum; basolateral plasma membrane; plasma membrane; integral to membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion