Human Claudin 8 ELISA Kit | CLDN8 elisa kit

Human Claudin 8 (CLDN8) ELISA Kit

Cat. Num. AAA2031916

• Gene Names: CLDN8; HEL-S-79
• Reactivity: Human
• Synonyms: Claudin 8; Human Claudin 8 (CLDN8) ELISA Kit; CLDN8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Claudin 8 (CLDN8).; No significant cross-reactivity or interference between Claudin 8 (CLDN8) and analogues was observed.
• Sequence Length: 225

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 8 (CLDN8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 8 (CLDN8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for CLDN8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 8 (CLDN8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 8 (CLDN8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 8 (CLDN8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 8 (CLDN8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CLDN8 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 40788011
• NCBI GeneID: 9073
• NCBI Accession #: NP_955360.1
• NCBI GenBank Nucleotide #: NM_199328.2
• Molecular Weight: 24,845 Da
• NCBI Official Full Name: claudin-8
• NCBI Official Synonym Full Names: claudin 8
• NCBI Official Symbol: CLDN8
• NCBI Official Synonym Symbols: HEL-S-79
• NCBI Protein Information: claudin-8
• UniProt Protein Name: Claudin-8
• Protein Family: Claudin
• UniProt Gene Name: CLDN8
• UniProt Entry Name: CLD8_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein plays important roles in the paracellular cation barrier of the distal renal tubule, and in the paracellular barrier to prevent sodium back-leakage in distal colon. Differential expression of this gene has been observed in colorectal carcinoma and renal cell tumors, and along with claudin-7, is an immunohistochemical marker for the differential diagnosis of chromophobe renal cell carcinoma and renal oncocytoma.[provided by RefSeq, May 2010]

Uniprot Description

Claudin-8: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Cell adhesion; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: apicolateral plasma membrane; basolateral plasma membrane; endoplasmic reticulum; integral to membrane; plasma membrane; tight junction

Molecular Function: identical protein binding; protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion