Mouse CD80 Monoclonal Antibody | anti-CD80 antibody

MOUSE ANTI HUMAN CD80:Preservative Free

Cat. Num. AAA210200

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: CD80; Monoclonal Antibody; MOUSE ANTI HUMAN CD80:Preservative Free; anti-CD80 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MEM-233
• Form/Format: Preservative Free; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-CD80 antibody: Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. ; Western Blotting: Maximum Dilution: Non-reducing conditions
• Preparation: Purified IgG prepared by affinity chromatography on Protein A
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of Daudi B cells with Mouse anti Human CD80:FITC (MBS210835))

Testing Data

(Staining of Daudi B cells with Mouse anti Human CD80:Alexa Fluor 647 (MBS212473A647))

Related Product Information for anti-CD80 antibody

Mouse anti Human CD80 antibody, clone MEM-233 recognizes human CD80, also known as B7-1, a ~60kDa type 1 trans-membrane protein expressed of macrophages, dendritic cells (Munro et al. 1994) and activated B-cells (Ranheim et al. 1993) CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33kDa, the presence of multiple (8) potential N-glycosylation sites (Chen et al. 1998) results in a migration corresponding to ~60kDa. Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 (Azuma et al. 1993) involved in T cell activation and proliferation (Vasu et al. 2003). Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics (van der Merwe et al. 1997). Site mutagenesis studies indicate residues in both the Ig-v-like and Ig-c-like domains of CD80 are crucial for the interaction with it's receptors CTLA-4 and CD28 (Peach et al. 1995). Mouse anti human CD80 antibody, clone MEM-233 binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching studies (Vasu et al. 2003). Functional studies using Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 suggest that clone MEM-233 is able to block binding of human CD80 with it's cognate ligands CD28 and CTLA-4 (Morbach et al. 2011).

NCBI and Uniprot Product Information

• NCBI GI #: 4885123
• NCBI GeneID: 941
• NCBI Accession #: NP_005182.1
• NCBI GenBank Nucleotide #: NM_005191.3
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: 18,761 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80; B-lymphocyte activation antigen B7; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CTLA-4 counter-receptor B7.1; activation B7-1 antigen; costimulatory factor CD80; costimulatory molecule variant IgV-CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7
• UniProt Entry Name: CD80_HUMAN

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 3q13.3-q21

Cellular Component: cell surface; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; viral reproduction; T cell activation; nerve growth factor receptor signaling pathway; positive regulation of signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of T-helper 1 cell differentiation