Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Carnitine-acylcarnitine translocase (CACT) Standard Curve.)
Human Carnitine-Acylcarnitine Translocase (CACT) ELISA Kit | CACT elisa kit
Human Carnitine-Acylcarnitine Translocase (CACT) ELISA Kit
Gene Names
SLC25A20; CAC; CACT
Reactivity
Human
Synonyms
Carnitine-Acylcarnitine Translocase (CACT); Human Carnitine-Acylcarnitine Translocase (CACT) ELISA Kit; SLC25A20; CAC; CACT; carnitine/acylcarnitine carrier protein; carnitine/acylcarnitine translocase; CACT elisa kit
Reactivity
Human
Specificity
Human Carnitine-acylcarnitine translocase (CACT) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC25A20. No significant cross-reactivity or interference between Human SLC25A20 and analogues was observed.
Sequence Length
301
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Carnitine-acylcarnitine translocase (CACT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Carnitine-acylcarnitine translocase (CACT) Standard Curve.)
Related Product Information for CACT elisa kit
Description: This Human Carnitine-acylcarnitine translocase (CACT) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC25A20 in samples. An antibody specific for SLC25A20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC25A20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC25A20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC25A20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Carnitine-acylcarnitine translocase is one of several closely related mitochondrial-membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. This protein mediates the transport of acylcarnitines into mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway. Mutations in this gene are associated with carnitine-acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
Background: Carnitine-acylcarnitine translocase is one of several closely related mitochondrial-membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. This protein mediates the transport of acylcarnitines into mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway. Mutations in this gene are associated with carnitine-acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitochondrial carnitine/acylcarnitine carrier protein
NCBI Official Synonym Full Names
solute carrier family 25 member 20
NCBI Official Symbol
SLC25A20
NCBI Official Synonym Symbols
CAC; CACT
NCBI Protein Information
mitochondrial carnitine/acylcarnitine carrier protein
UniProt Protein Name
Mitochondrial carnitine/acylcarnitine carrier protein
Protein Family
UniProt Gene Name
SLC25A20
UniProt Synonym Gene Names
CAC; CACT; CAC
UniProt Entry Name
MCAT_HUMAN
NCBI Description
This gene product is one of several closely related mitochondrial-membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. This protein mediates the transport of acylcarnitines into mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway. Mutations in this gene are associated with carnitine-acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Mediates the transport of acylcarnitines of different length across the mitochondrial inner membrane from the cytosol to the mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway.
Research Articles on CACT
Similar Products
Product Notes
The Human CACT slc25a20 (Catalog #AAA9714277) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714277 ELISA Kit recognizes Human CACT. It is sometimes possible for the material contained within the vial of "Carnitine-Acylcarnitine Translocase (CACT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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