Rabbit anti-Human MARCO Polyclonal Antibody | anti-MARCO antibody

MARCO Antibody (N-term)

Cat. Num. AAA9206180

• Gene Names: MARCO; SCARA2
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MARCO; Polyclonal Antibody; MARCO Antibody (N-term); Macrophage receptor MARCO; Macrophage receptor with collagenous structure; Scavenger receptor class A member 2; SCARA2; anti-MARCO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This MARCO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human MARCO.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 13-40
• Sequence Length: 442

• Applicable Applications for anti-MARCO antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry - Paraffin (IHC-P)
• Application Notes: WB~~1:1000; FC~~1:10~50; IHC-P~~1:50~100
• Function: Pattern recognition receptor (PRR) which binds Gram- positive and Gram-negative bacteria (PubMed:9468508). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (PubMed:12847263).
• Cellular Location: Cell membrane; Single-pass type II membrane protein
• Tissue Location: Expressed in alveolar macrophages (at protein level). Detected in macrophages from various tissues including thymus, kidney, Kupffer cells of liver, and spleen (PubMed:9468508).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-MARCO Antibody (N-term) at 1:500 dilution + HACAT whole cell lysateLysates/proteins at 20 µg per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Flow Cytometry (FC/FACS)

(MARCO Antibody (N-term) (MBS9206180) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(MARCO Antibody (N-term) (MBS9206180) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsillitis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MARCO Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MARCO antibody

MARCO is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.

Product Categories/Family for anti-MARCO antibody

Immunology; Microbiology

References

Wright, A.K., et al. J. Leukoc. Biol. 86(3):479-489(2009)
Trynka, G., et al. Gut 58(8):1078-1083(2009)
Arredouani, M.S., et al. J. Immunol. 175(9):6058-6064(2005)
Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005)
Seta, N., et al. Arthritis Rheum. 44(4):931-939(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 5803080
• NCBI GeneID: 8685
• NCBI Accession #: NP_006761.1
• NCBI GenBank Nucleotide #: NM_006770.3
• UniProt Accession #: Q9UEW3; Q9Y5S3; B4DW79
• NCBI Official Full Name: macrophage receptor MARCO
• NCBI Official Synonym Full Names: macrophage receptor with collagenous structure
• NCBI Official Symbol: MARCO
• NCBI Official Synonym Symbols: SCARA2
• NCBI Protein Information: macrophage receptor MARCO
• UniProt Protein Name: Macrophage receptor MARCO
• Protein Family: Macrophage receptor
• UniProt Gene Name: MARCO
• UniProt Synonym Gene Names: SCARA2
• UniProt Entry Name: MARCO_HUMAN

NCBI Description

The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. [provided by RefSeq, Jul 2008]

Uniprot Description

MARCO: Pattern recognition receptor (PRR). Binds Gram-positive and Gram-negative bacteria.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: collagen; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; scavenger receptor activity; pattern recognition receptor activity

Biological Process: receptor-mediated endocytosis; cell surface receptor linked signal transduction; innate immune response; pattern recognition receptor signaling pathway; apoptotic cell clearance