Human Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) ELISA Kit | BCAR3 elisa kit

Human Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) ELISA Kit

Cat. Num. AAA2025100

• Gene Names: BCAR3; NSP2; SH2D3B
• Reactivity: Human
• Synonyms: Breast Cancer Anti-Estrogen Resistance 3 (BCAR3); Human Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) ELISA Kit; NSP2; SH2D3B; Novel SH2-containing protein 2; SH2 domain-containing protein 3B; BCAR3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Breast Cancer Anti-Estrogen Resistance 3 (BCAR3).; No significant cross-reactivity or interference between Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) and analogues was observed.
• Sequence Length: 825

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 78.12-5000pg/mL
• Sensitivity: < 27pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCAR3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 3 (BCAR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 3 (BCAR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 3 (BCAR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BCAR3 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 387157909
• NCBI GeneID: 8412
• NCBI Accession #: NP_001248338.1
• NCBI GenBank Nucleotide #: NM_001261409.1
• Molecular Weight: 82,357 Da
• NCBI Official Full Name: breast cancer anti-estrogen resistance protein 3 isoform 1
• NCBI Official Synonym Full Names: breast cancer anti-estrogen resistance 3
• NCBI Official Symbol: BCAR3
• NCBI Official Synonym Symbols: NSP2; SH2D3B
• NCBI Protein Information: breast cancer anti-estrogen resistance protein 3
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 3
• Protein Family: Breast cancer anti-estrogen resistance protein
• UniProt Gene Name: BCAR3
• UniProt Synonym Gene Names: NSP2; SH2D3B
• UniProt Entry Name: BCAR3_HUMAN

NCBI Description

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

BCAR3: a ubiquitously expressed regulator of R-Ras activity that has been implicated in breast cancer. A GDP exchange factor whose overexpression confers antiestrogen resistance, activates Rac, PAK1, and the cyclin D1. Can accelerate the degradation of IRS-1, thus regulating signaling through the insulin receptor. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. Up-regulated by interleukin-1-alpha and TNF-beta in thymus cortical reticular cell lines (mouse).Two alternatively spliced human isoforms have been described.

Protein type: GEFs, Ras; Adaptor/scaffold; Motility/polarity/chemotaxis; GEFs

Chromosomal Location of Human Ortholog: 1p22.1

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity

Biological Process: response to drug; small GTPase mediated signal transduction; lens morphogenesis in camera-type eye; signal transduction; positive regulation of peptidyl-serine phosphorylation; positive regulation of GTPase activity