Breast Cancer Anti-Estrogen Resistance 3 Recombinant Protein | BCAR3 recombinant protein

Recombinant Breast Cancer Anti-Estrogen Resistance 3 (BCAR3)

Cat. Num. AAA2029286

• Gene Names: BCAR3; NSP2; SH2D3B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Breast Cancer Anti-Estrogen Resistance 3; Recombinant Breast Cancer Anti-Estrogen Resistance 3 (BCAR3); BCAR3 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; DPKVIAQHVLSMD CRVARILGVS EEMRRNMGVS SGLELITLPH GHQLRLDIIERHNTMAIGIA VDILGCTGTL EDRAATLSKI IQVAVELKDS MGDLYSFSALMKALEMPQIT RLEKTWTALR HQYTQTAILY EKQLKPFSKL LHEGRESTCVPPNNVSVPLL MPLVTLMERQ AVTFEGTDMW EKNDQSCEIM LNHLATARFMAEAADSYRMN AERILAGFQP DEEMNEICKT EFQMRLLWGS KGAQVNQTERYEKFNQILTA LSRKLEPP
• Sequence Length: 825

• Applicable Applications for BCAR3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 60.7kDa
• Accurate Molecular Mass (KD): 61kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Asp548~Pro818
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 387157909
• NCBI GeneID: 8412
• NCBI Accession #: NP_001248338.1
• NCBI GenBank Nucleotide #: NM_001261409.1
• Molecular Weight: 82,357 Da
• NCBI Official Full Name: breast cancer anti-estrogen resistance protein 3 isoform 1
• NCBI Official Synonym Full Names: breast cancer anti-estrogen resistance 3
• NCBI Official Symbol: BCAR3
• NCBI Official Synonym Symbols: NSP2; SH2D3B
• NCBI Protein Information: breast cancer anti-estrogen resistance protein 3
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 3
• Protein Family: Breast cancer anti-estrogen resistance protein
• UniProt Gene Name: BCAR3
• UniProt Synonym Gene Names: NSP2; SH2D3B
• UniProt Entry Name: BCAR3_HUMAN

NCBI Description

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

BCAR3: a ubiquitously expressed regulator of R-Ras activity that has been implicated in breast cancer. A GDP exchange factor whose overexpression confers antiestrogen resistance, activates Rac, PAK1, and the cyclin D1. Can accelerate the degradation of IRS-1, thus regulating signaling through the insulin receptor. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. Up-regulated by interleukin-1-alpha and TNF-beta in thymus cortical reticular cell lines (mouse).Two alternatively spliced human isoforms have been described.

Protein type: Adaptor/scaffold; GEFs; Motility/polarity/chemotaxis; GEFs, Ras

Chromosomal Location of Human Ortholog: 1p22.1

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity

Biological Process: response to drug; small GTPase mediated signal transduction; lens morphogenesis in camera-type eye; signal transduction; positive regulation of peptidyl-serine phosphorylation; positive regulation of GTPase activity