Mouse Breast cancer anti-estrogen resistance protein 3 ELISA Kit | Bcar3 elisa kit

Mouse Breast cancer anti-estrogen resistance protein 3 ELISA Kit

Cat. Num. AAA760455

• Gene Names: BCAR3; NSP2; SH2D3B
• Reactivity: Mouse
• Synonyms: Breast cancer anti-estrogen resistance protein 3; Mouse Breast cancer anti-estrogen resistance protein 3 ELISA Kit; Bcar3/And34/Breast cancer anti-estrogen resistance protein 3/p130Cas-binding protein AND-34; Bcar3 elisa kit

• Reactivity: Mouse
• Sequence Length: 825

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Bcar3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Bcar3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Bcar3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Bcar3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Bcar3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 387157909
• NCBI GeneID: 8412
• NCBI Accession #: NP_001248338.1
• NCBI GenBank Nucleotide #: NM_001261409.1
• Molecular Weight: 82,357 Da
• NCBI Official Full Name: breast cancer anti-estrogen resistance protein 3 isoform 1
• NCBI Official Synonym Full Names: breast cancer anti-estrogen resistance 3
• NCBI Official Symbol: BCAR3
• NCBI Official Synonym Symbols: NSP2; SH2D3B
• NCBI Protein Information: breast cancer anti-estrogen resistance protein 3
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 3
• Protein Family: Breast cancer anti-estrogen resistance protein
• UniProt Gene Name: BCAR3
• UniProt Synonym Gene Names: NSP2; SH2D3B
• UniProt Entry Name: BCAR3_HUMAN

NCBI Description

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

BCAR3: a ubiquitously expressed regulator of R-Ras activity that has been implicated in breast cancer. A GDP exchange factor whose overexpression confers antiestrogen resistance, activates Rac, PAK1, and the cyclin D1. Can accelerate the degradation of IRS-1, thus regulating signaling through the insulin receptor. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. Up-regulated by interleukin-1-alpha and TNF-beta in thymus cortical reticular cell lines (mouse).Two alternatively spliced human isoforms have been described.

Protein type: GEFs, Ras; Adaptor/scaffold; Motility/polarity/chemotaxis; GEFs

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: intracellular

Molecular Function: guanyl-nucleotide exchange factor activity; protein binding

Biological Process: lens morphogenesis in camera-type eye; positive regulation of GTPase activity; positive regulation of peptidyl-serine phosphorylation; response to drug; signal transduction; small GTPase mediated signal transduction