Rat ciliary neurotrophic factor ELISA Kit | CNTF elisa kit

Rat Ciliary Neurotrophic Factor, CNTF ELISA Kit

Cat. Num. AAA704925

• Reactivity: Rat
• Synonyms: ciliary neurotrophic factor; Rat Ciliary Neurotrophic Factor; CNTF ELISA Kit; HCNTF; ; CNTF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat CNTF. No significant cross-reactivity or interference between Rat CNTF and analogues was observed.
• Sequence Length: 199

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Differentiation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTF elisa kit

Introduction: Ciliary neurotrophic factor (CNTF), is a polypeptide hormone and nerve growth factor whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations including astrocytes. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CNTF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CNTF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CNTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CNTF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126723183
• NCBI GeneID: 100009117
• NCBI Accession #: NP_001075752.1
• NCBI GenBank Nucleotide #: NM_001082283.1
• UniProt Accession #: P14188
• Molecular Weight: 22,662 Da
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Symbol: CNTF
• NCBI Protein Information: ciliary neurotrophic factor; ciliary neurotropic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: CNTF
• UniProt Synonym Gene Names: CNTF
• UniProt Entry Name: CNTF_RABIT

Uniprot Description

Function: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.