Rabbit anti-Human KRR1 Polyclonal Antibody | anti-KRR1 antibody

KRR1, NT (KRR1, HRB2, KRR1 small subunit processome component homolog, HIV-1 Rev-binding protein 2, KRR-R motif-containing protein 1, Rev-interacting protein 1) (Azide free) (HRP)

Cat. Num. AAA6314528

• Gene Names: KRR1; HRB2; RIP-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KRR1; Polyclonal Antibody; NT (KRR1; HRB2; KRR1 small subunit processome component homolog; HIV-1 Rev-binding protein 2; KRR-R motif-containing protein 1; Rev-interacting protein 1) (Azide free) (HRP); Rev-interacting protein 1; anti-KRR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-KRR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KRR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human KRR1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KRR1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KRR1 antibody detected the KRR1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of KRR1 (arrow) using rabbit polyclonal KRR1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KRR1 gene.)

Related Product Information for anti-KRR1 antibody

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity).

Product Categories/Family for anti-KRR1 antibody

Antibodies; Infectious Disease HIV

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 117676402
• NCBI GeneID: 11103
• NCBI Accession #: NP_008974.5
• NCBI GenBank Nucleotide #: NM_007043
• UniProt Accession #: Q13601; Q8NEA8; Q8TC37; Q96AT5; A0FIK6; A0JLP0; B2R989; E7EUQ0
• Molecular Weight: 36,788 Da
• NCBI Official Full Name: Homo sapiens KRR1, small subunit (SSU) processome component, homolog (yeast) (KRR1), mRNA
• NCBI Official Synonym Full Names: KRR1, small subunit (SSU) processome component, homolog (yeast)
• NCBI Official Symbol: KRR1
• NCBI Official Synonym Symbols: HRB2; RIP-1
• NCBI Protein Information: KRR1 small subunit processome component homolog; HIV-1 Rev binding protein 2; HIV-1 Rev-binding protein 2; KRR-R motif-containing protein 1; Rev interacting protein; rev-interacting protein 1
• UniProt Protein Name: KRR1 small subunit processome component homolog
• Protein Family: KRR1 small subunit processome component
• UniProt Gene Name: KRR1
• UniProt Synonym Gene Names: HRB2; Rip-1
• UniProt Entry Name: KRR1_HUMAN

Uniprot Description

KRR1: Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Belongs to the KRR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA processing

Chromosomal Location of Human Ortholog: 12q21.2

Cellular Component: small subunit processome; intercellular bridge; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)