Human Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit | BMP8B elisa kit

Human Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit

Cat. Num. AAA2704178

• Gene Names: BMP8B; OP2; BMP8
• Reactivity: Human
• Synonyms: Bone Morphogenetic Protein 8B (BMP8B); Human Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit; BMP8-B; OP2; Osteogenic Protein 2; BMP8B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 8B (BMP8B).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 8B (BMP8B) and analogues was observed.
• Sequence Length: 349

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 7.0pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 8B (BMP8B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 8B (BMP8B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP8B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 8B (BMP8B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 8B (BMP8B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 8B (BMP8B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 8B (BMP8B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32250692
• NCBI GeneID: 656
• Molecular Weight: 39,151 Da
• NCBI Official Full Name: bone morphogenetic protein 8B
• NCBI Official Synonym Full Names: bone morphogenetic protein 8b
• NCBI Official Symbol: BMP8B
• NCBI Official Synonym Symbols: OP2; BMP8
• NCBI Protein Information: bone morphogenetic protein 8B
• UniProt Protein Name: Bone morphogenetic protein 8B
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP8B
• UniProt Synonym Gene Names: BMP8; BMP-8; BMP-8B; OP-2

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The encoded protein stimulates thermogenesis in brown adipose tissue. Expression of this gene may be downregulated in pancreatic cancer. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis ().