Typical Testing Data/Standard Curve (for reference only)
Mouse Bone morphogenetic protein 8B (BMP8B) ELISA Kit | BMP8B elisa kit
Mouse Bone morphogenetic protein 8B (BMP8B) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BMP-8B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP-8B antibody and an BMP-8B-HRP conjugate. The assay sample and buffer are incubated together with BMP-8B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP-8B concentration since BMP-8B from samples and BMP-8B-HRP conjugate compete for the anti-BMP-8B antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP-8B from the sample, fewer sites are left to bind BMP-8B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP-8B concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity. [provided by RefSeq, Jul 2008]
Uniprot Description
BMP8B: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Belongs to the TGF-beta family.
Protein type: Secreted; Secreted, signal peptide; Cytokine
Chromosomal Location of Human Ortholog: 1p35-p32
Cellular Component: extracellular space
Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding
Biological Process: BMP signaling pathway; regulation of apoptosis; ossification; cartilage development; regulation of MAPKKK cascade; skeletal development; cell development; growth
Research Articles on BMP8B
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Product Notes
The Mouse BMP8B bmp8b (Catalog #AAA7245115) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7245115 ELISA Kit recognizes Mouse BMP8B. It is sometimes possible for the material contained within the vial of "Bone morphogenetic protein 8B (BMP8B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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